GLYCOPROTEINS OF BOVINE VIRAL DIARRHEA-MUCOSAL DISEASE VIRUS IN INFECTED BOVINE CELLS
GLYCOPROTEINS OF BOVINE VIRAL DIARRHEA-MUCOSAL DISEASE VIRUS IN INFECTED BOVINE CELLS
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DOI：
10.1099/0022-1317-68-6-1607
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发表时间：
1987-06-01
影响因子：
3.8
通讯作者：
DUBOVI, EJ
中科院分区：
文献类型：
--
作者：
DONIS, RO;DUBOVI, EJ
用L-[35 S]甲硫氨酸或D[2- 3 H]甘露糖对感染牛病毒性腹泻-粘膜病（BD）病毒的牛细胞培养物进行放射性标记，然后通过放射免疫沉淀和聚丙烯酰胺凝胶电泳在一维和二维上分析标记的多肽。在感染细胞中检测到6种糖蛋白。两种丰度较高的物种的Mr值分别为48 K和56 K ~ 58 K，丰度较低的物种的Mr值分别为118 K、75 K、65 K和5 K。当细胞用L-[35 S]甲硫氨酸放射性标记时，在存在被膜素的情况下，56 K至58 K在PAGE中迁移，表观质量为54 K（次要分子种类）和48 K至50 K（主要分子种类）。内切糖苷F消化病毒感染的多肽导致4K至6 K的表观分子量的56 K至58 K的减少，产生单一的52 K定向产物，表明56 K至58 K的异质性是由于寡糖部分的差异。衣霉素导致感染性病毒产量急剧降低，表明碳水化合物部分在BVD病毒的感染循环中起关键作用。
Bovine cell cultures infected with bovine viral diarrhoea-mucosal disease (BD) virus were radiolabelled with L-[35S]methionine or D[2-3H]mannose followed by analysis of the labelled polypeptides by radioimmunoprecipitation and polyacrylamide gel electrophoresis in one and two dimensions. Six glycoproteins were detected infected cells. Two abundant species had Mr of 48K and 56K to 58K while the less abundant species had Mr of 118K, 75K, 65K and 5K. When cells were radiolabelled with L-[35S]methionine in the presence of tunicymin 56K to 58K migrated with apparent masses of 54K (a minor species) and 48K to 50K (the major molecular species) in PAGE. Endoglycosides F digestion of virus-infected polypeptides caused 4K to 6K reduction in the apparent molecular mass of 56K to 58K yielding a single 52K direted product, indicating that the heterogeneity of 56K to 58K was due to differences in the oligosaccharide moieties. Tunicamycin caused a drastic reduction in the yield of infectious virus indicating that the carbohydrate moieties serve a critical role in the infectious cycle of BVD virus.