Genetical survey of novel type of Cryptosporidium andersoni in cattle in Japan.
Genetical survey of novel type of Cryptosporidium andersoni in cattle in Japan.
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DOI：
10.1016/j.vetpar.2008.08.021
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发表时间：
2008-11
影响因子：
2.6
通讯作者：
M. Matsubayashi;Saki Nagano;T. Kita;T. Narushima;I. Kimata;M. Iseki;Tomoya Hajiri;H. Tani;K. Sasai;E. Baba
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文献类型：
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作者：
M. Matsubayashi;Saki Nagano;T. Kita;T. Narushima;I. Kimata;M. Iseki;Tomoya Hajiri;H. Tani;K. Sasai;E. Baba
以前，我们报道了在日本牛中检测到的一种新型安氏隐孢子虫分离株含有A型（与C. andersoni先前报道）和B型（具有不同于A型的胸腺嘧啶核苷酸插入）基因型。在这里，我们进行了广泛的调查隐孢子虫感染成年牛在日本从2004年至2007年。因此，在205头牛中有12头（5.9%）检出隐孢子虫，所有分离株的隐孢子虫卵囊壁蛋白（COWP）基因的部分序列与先前报道的隐孢子虫的数据相同。andersoni的18 S rRNA基因部分序列中均观察到两个信号。在使用其中五个分离株的传播研究中，它们都感染了SCID小鼠。对分离自SCID小鼠的单个卵囊进行多重PCR检测，发现10株分离株18 SrRNA基因部分序列与A型完全相同，占40-80%。andersoni和其他样品的那些与B型基因型相同。这些结果表明，C. andersoni新类型在整个日本的牛中广泛存在，并且在18 S rRNA基因中具有多个拷贝（A型和B型）。
Previously, we reported that an isolate of novel type of Cryptosporidium andersoni detected in cattle in Japan contained Type A (identical to C. andersoni reported previously) and Type B (having a thymine nucleotide insertion unlike the Type A) genotypes in the 18S rRNA gene. Here, we conducted an extensive investigation of Cryptosporidium infections in adult cattle in Japan from 2004 to 2007. Consequently, Cryptosporidium sp. were detected in 12 of the 205 cattle examined (5.9%), and partial sequences of the Cryptosporidium oocyst wall protein (COWP) gene in all isolates were identical to those of the previously reported data for C. andersoni whereas two signals were observed in the sequence of the partial 18S rRNA gene in all the isolates. In transmission studies using five of the isolates, they all infected SCID mice. Modified multiplex PCR using DNA of a single oocyst isolated from the infected SCID mice revealed that the partial sequences in the 18S rRNA gene of 40–80% of 10 isolates were identical to the Type A genotype of C. andersoni and those of other samples were identical to the Type B genotype. These results suggested that the C. andersoni novel type is widespread in cattle throughout Japan, and have multiple copies (Types A and B) in the 18S rRNA gene.