Antioxidative potential of folate producing probiotic Lactobacillus helveticus CD6
Antioxidative potential of folate producing probiotic Lactobacillus helveticus CD6
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DOI：
10.1007/s13197-011-0244-0
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发表时间：
2013-02-01
影响因子：
3.1
通讯作者：
Chaudhari, Bhushan Liladhar
中科院分区：
文献类型：
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作者：
Ahire, Jayesh Jagannath;Mokashe, Narendra Uttamrao;Chaudhari, Bhushan Liladhar
产叶酸乳杆菌。16S rRNA基因序列分析表明，从发酵乳中分离到的CD6与瑞士乳杆菌有98%的相似性。发现它能产生叶酸衍生物5-甲基四氢叶酸(5-MeTHF)。菌株的胞内无细胞提取物具有抗氧化活性，对抗坏血酸自氧化的抑制率为27.5%+/-3.7%。与Cu2+相比，Fe2+对金属离子的络合能力最强(0.26+/-0.06ppm)。对完整细胞的DPPH(α，α-二苯基-β-苦皮酰肼)自由基清除能力为24.7%+/-10.9%，证明了其抗氧化能力。对肾上腺素自氧化的抑制率为14.89%，半胱氨酸当量还原活性为20.9+/-1.8 mU/g，清除羟基自由基活性为20.8%/-0.9%。根据世卫组织和粮农组织的指南对该菌株的益生菌特性进行了评估。在高酸性条件下(pH 2.0)存活率为90.61%，在人工胃液中存活率为90.66%，在0.5%胆汁浓度下24 h存活率为68%，对多种抗生素敏感，降低了以益生菌制剂形式给其他细菌提供耐药性决定簇的可能性。在体外对鼠伤寒沙门氏菌(NCIM 2501)、化脓性链球菌(NCIM 2608)、金黄色葡萄球菌(NCIM 5021)等肠道致病菌具有粘液结合和抗菌活性，对绵羊血琼脂无溶血活性。
Folate producing Lactobacillus sp. CD6 isolated from fermented milk showed 98% similarity with Lactobacillus helveticus based on 16S rRNA gene sequence analysis. It was found to produce a folic acid derivative 5-methyl tetrahydrofolate (5-MeTHF). The intracellular cell-free extract of strain demonstrated antioxidative activity with the inhibition rate of ascorbate autoxidation in the range of 27.5% +/- 3.7%. It showed highest metal ion chelation ability for Fe2+ (0.26 +/- 0.06 ppm) as compared to Cu2+. The DPPH (alpha,alpha-Diphenyl-beta-Picrylhydrazyl) radical scavenging activity for intact cells were found to be 24.7% +/- 10.9% proved its antioxidative potential. Furthermore, it demonstrated 14.89% inhibition of epinephrine autoxidation, 20.9 +/- 1.8 mu g cysteine equivalent reducing activity and 20.8% +/- 0.9% hydroxyl radical scavenging effect. The strain was evaluated for probiotic properties as per WHO and FAO guidelines. It showed 90.61% survival at highly acidic condition (pH 2.0), 90.66% viability in presence of synthetic gastric juice and 68% survivability at 0.5% bile concentration for 24 h. It was susceptible to many antibiotics which reduces the prospect to offer resistance determinants to other organisms if administered in the form of probiotic preparations. It showed in vitro mucus binding and antimicrobial activity against enteric pathogens like Salmonella typhimurium (NCIM 2501), Streptococcus pyogenes (NCIM 2608), and Staphylococcus aureus (NCIM 5021) and moreover it showed non- hemolytic activity on sheep blood agar.