Solochrome cyanine R as an indicator dye of bone morphology.
Solochrome cyanine R as an indicator dye of bone morphology.
复制标题
Solochrome 花青 R 作为骨形态指示剂染料。
作者：
H. Matrajt;D. Hioco
将未矿化骨切片包埋在聚酯树脂中并以6 μ m切割，在不去除包埋基质的情况下，在由Solochrome花青R（1 g/l）、冰醋酸（2 ml）和蒸馏水（98 ml）组成的水溶液中染色10 min。通过乙酸获得约2的pH。将切片在30 ℃的自来水中洗涤并区分，在上升的醇中脱水，清洁并固定在合成树脂中。“年轻的类骨质”染成浅橙子，在类骨质接缝的其余部分，可以区分两种类型的薄层：一层蓝色的基质或胶原蛋白层和一层橙子的纤维状胶原蛋白层。“钙化前沿”以深蓝色清晰地划分出来。
Sections of undemineralized bone embedded in a polyester resin and cut at 6 μ are stained for 10 min, without removal of the embedding matrix, in an aqueous solution composed of Solochrome cyanine R, 1 gin; glacial acetic acid, 2 ml; and distilled water, 98 ml. A pH about 2 is obtained by the acetic acid. The sections are washed and differentiated in tap water at 30 C, dehydrated in ascending alcohols, cleared and mounted in synthetic resin. “Young osteoid” stains light orange and, in the rest of an osteoid seam, two types of lamellae can be distinguished: one blue layer of ground substance or collagen and one orange layer of fibrillar collagen. The “calcification front” is sharply demarcated by its dark blue color.