The dual role of the potyvirus P3 protein of turnip mosaic virus as a symptom and avirulence determinant in brassicas
The dual role of the potyvirus P3 protein of turnip mosaic virus as a symptom and avirulence determinant in brassicas
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DOI：
10.1094/mpmi.2003.16.9.777
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发表时间：
2003-09-01
影响因子：
3.5
通讯作者：
Walsh, JA
中科院分区：
文献类型：
--
作者：
Jenner, CE;Wang, XW;Walsh, JA
芜菁花叶病毒（TuMV）的两个分离株UK 1和CDN 1在易感繁殖宿主芥菜（Brassica juncea）上的一般症状和感染具有多种显性抗性基因的甘蓝型油菜品系的能力不同。分离物CDN 1在受感染的芸苔叶片中产生比UK 1更极端的花叶，并且能够克服TuRB01， TuRB04和TuRB05抗性基因。甘蓝型油菜22S的抗性基因TuRB03对CDN 1有效，但对uk1无效。uk1与CDN 1分离株的核酸序列有90%相同。P3蛋白的c端一半被确定为juncea症状差异的原因。发现P3蛋白中的一个氨基酸是TuRB03的无毒决定因素。先前的工作已经确定P3是TuRB04的无毒决定因素。我们的研究结果增加了对植物病毒识别基础的理解，显示了多病毒P3基因作为症状决定因素的重要性，并为植物正常感染周期中鲜为人知的P3蛋白提供了作用。
Two isolates of the potyvirus Turnip mosaic virus (TuMV), UK 1 and CDN 1, differ both in their general symptoms on the susceptible propagation host Brassica juncea and in their ability to infect B. napus lines possessing a variety of dominant resistance genes. The isolate CDN 1 produces a more extreme mosaic in infected brassica leaves than UK 1 and is able to overcome the resistance genes TuRB01, TuRB04, and TuRB05. The resistance gene TuRB03, in the B. napus line 22S, is effective against CDN 1 but not UK 1. The nucleic acid sequences of the UK 1 and CDN 1 isolates were 90% identical. The C-terminal half of the P3 protein was identified as being responsible for the differences in symptoms in B. juncea. A single amino acid in the P3 protein was found to be the avirulence determinant for TuRB03. Previous work already has identified the P3 as an avirulence determinant for TuRB04. Our results increase the understanding of the basis of plant-virus recognition, show the importance of the potyviral P3 gene as a symptom determinant, and provide a role in planta for the poorly understood P3 protein in a normal infection cycle.