The response of gut-associated T lymphocytes to intestinal viral immunization.
The response of gut-associated T lymphocytes to intestinal viral immunization.
复制标题
肠道相关 T 淋巴细胞对肠道病毒免疫的反应。
作者：
T. Issekutz
肠道相关淋巴组织对肠道内抗原的细胞免疫反应知之甚少。我们的目的是研究痘苗病毒肠内免疫后GALT细胞的抗原特异性T细胞增殖反应和细胞毒性T淋巴细胞(CTL)反应。小肠腔内注射痘苗病毒6天后，在肠系膜淋巴结(MLN)内发现大量能在痘苗病毒存在下增殖的淋巴细胞。此时的MLN也含有痘苗病毒特异性的CTL，但与免疫后几周内发现的增殖细胞不同，MLN中的CTL仅在几天内可见。Peyer‘s斑块既不含抗原刺激的增殖细胞，也不含CTL。免疫后10d，病毒特异性增殖淋巴细胞为SIG-、单抗W3/25+、MRC OX-8-大淋巴母细胞。痘苗病毒特异性CTL也是大淋巴母细胞，但它们属于W3/25-，OX-8+亚群。因此，病毒攻击肠道后，在MLN内产生强烈的T辅助和细胞毒性T淋巴母细胞反应。
The cell-mediated immune response by the gut-associated lymphoid tissues to antigens within the intestinal tract is poorly understood. Our objective was to investigate the antigen-specific T cell proliferative response and cytotoxic T lymphocyte (CTL) response of cells from the GALT after enteric immunization with vaccinia virus. Lymphocytes able to proliferate in the presence of vaccinia virus in vitro were found in large numbers in mesenteric lymph nodes (MLN) 6 days after the injection of vaccinia virus into the lumen of the small bowel. The MLN at this time also contained vaccinia-specific CTL, but unlike the proliferating cells, which were found for several weeks after immunization, the CTL were demonstrable in the MLN for only a few days. Peyer's patches were found to contain neither antigen-stimulated proliferating cells nor CTL. The viral-specific proliferating lymphocytes from the MLN 10 days after immunization were sIg-, monoclonal antibody W3/25+, MRC OX-8- large lymphoblasts. The vaccinia-specific CTL were also large lymphoblasts, but they belonged to the W3/25-, OX-8+ subset. Thus, a strong T helper and cytotoxic T lymphoblast response is generated within the MLN after viral challenge of the gut.