Methods to Assess Blood and Nectar Meals in Aedes aegypti Mosquitoes.
Methods to Assess Blood and Nectar Meals in Aedes aegypti Mosquitoes.
复制标题
评估埃及伊蚊血液和花蜜膳食的方法。
DOI：
10.1101/pdb.top107657
复制
发表时间：
2022
影响因子：
--
通讯作者：
Duvall,LauraB
中科院分区：
文献类型：
--
作者：
Venkataraman,Krithika;Jové,Veronica;Duvall,LauraB
雄性和雌性埃及伊蚊都以花蜜为食以获取代谢能量，但雌性蚊子则需要通过叮咬宿主获得血液蛋白来产卵。虽然雄性只从花蜜中获取能量，但雌性必须选择最适合它们目前代谢和繁殖需要的食物。在雌性中，血液和花蜜促进独立的摄食行为，具有不同的感觉附属物、食量、消化道目标和代谢命运。了解雄性和雌性蚊子如何识别、定位和代谢营养物质，对于描述这种蚊子的生存和繁殖能力至关重要。在这里，我们介绍了血液与花蜜的摄食，以及量化个体花蜜和血粉大小的方法。蚊。精确量化食物的大小对于确保记录摄食行为下游事件的一致性至关重要，包括吸引宿主或繁殖力。
Male and female Aedes aegypti mosquitoes survive by feeding on floral nectar for metabolic energy, but females require blood protein, obtained from biting a host, for egg development. Although males exclusively derive energy from nectar sugars, females must select the meal that best matches their present metabolic and reproductive needs. In females, blood and nectar promote independent feeding behaviors with distinct sensory appendages, meal sizes, digestive tract targets, and metabolic fates. Understanding how male and female mosquitoes recognize, locate, and metabolize nutrients is essential for characterizing the survival and reproductive capabilities of this mosquito. Here, we provide an introduction to blood versus nectar feeding and methods to quantify nectar and blood meal sizes in individual Ae. aegypti mosquitoes. Precise quantification of meal size is crucial for ensuring consistency in assays that record events downstream of feeding behavior, including host attraction or fecundity.