SpineJ: A software tool for quantitative analysis of nanoscale spine morphology
SpineJ: A software tool for quantitative analysis of nanoscale spine morphology
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DOI：
10.1016/j.ymeth.2020.01.020
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发表时间：
2020-03-01
期刊：
影响因子：
4.8
通讯作者：
Sibarita, Jean-Baptiste
中科院分区：
文献类型：
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作者：
Levet, Florian;Tonnesen, Jan;Sibarita, Jean-Baptiste
超分辨率显微镜为活体脑组织中神经元的复杂形态提供了无衍射限制的光学通道，解决了它们最细微的结构细节，这对神经元功能至关重要。然而，由于现有的图像分析软件工具已经被开发用于衍射限制的图像，它们通常不太适合于量化纳米级结构，如树突棘。我们提出SpineJ，一个专门为此目的设计的半自动ImageJ插件。SpineJ提供了一个直观和用户友好的图形用户界面，便于快速，准确和公正的脊柱形态分析。
Super-resolution microscopy provides diffraction-unlimited optical access to the intricate morphology of neurons in living brain tissue, resolving their finest structural details, which are critical for neuronal function. However, as existing image analysis software tools have been developed for diffraction-limited images, they are generally not well suited for quantifying nanoscale structures like dendritic spines. We present SpineJ, a semi-automatic ImageJ plugin that is specifically designed for this purpose. SpineJ offers an intuitive and user-friendly graphical user interface, facilitating fast, accurate, and unbiased analysis of spine morphology.