EXPERIMENTAL INITIATION OF HAUSTORIA IN AGALINIS PURPUREA (SCROPHULARIACEAE)
EXPERIMENTAL INITIATION OF HAUSTORIA IN AGALINIS PURPUREA (SCROPHULARIACEAE)
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紫花吸器（玄参科）的实验启动
DOI：
10.1002/j.1537-2197.1979.tb06259.x
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发表时间：
1979
影响因子：
3
通讯作者：
L. Musselman
中科院分区：
文献类型：
--
作者：
J. L. Riopel;L. Musselman
对玄参科紫珠菌吸器的发生和早期发育进行了研究。使植物在具有0.5%蔗糖补充物的0.9Wo琼脂无机培养基中生长。绢毛胡枝子根系分泌物诱导吸器的发生，最早期为6-12 h，30 min的分泌物处理使吸器发生频率增加，24 h的分泌物处理使吸器发生频率与对照植株连续5 d的根系分泌物处理的吸器发生频率相当。描述了吸器发育的早期细胞学特征，并讨论了吸器起始在寄主识别中的可能意义。紫盖螺Agalinis PURPUREA（L.）RAF.是玄参科的一种含叶绿素的根部寄生物。这植物是北美东部的一种广布的一年生草本植物。与Rhinanthoideae亚科的其他半寄生虫一样，它产生称为吸器的特化根结构，形成宿主和寄生虫之间的形态和生理桥梁。吸器结构已在许多研究中描述，但启动和发展的过程很少受到关注（Kuijt，1977）。关于吸器发育的唯一实验工作是Malcolm（1966）对Castilleja coccarium和Melampyrum lineare的研究，瑟曼（1966）对几种直果属植物的研究，以及Atsatt（1973）对直果属紫果的琼脂培养的报道。这些研究并没有集中在启动的细胞学细节。对亚洲独脚金（Rogers和纳尔逊，1962年）和佛氏刺蛾（Visser、Dorr和Kollmann，1977年）早期寄生虫接触的发育观察表明，在接触宿主后，根尖直接转化为吸器。最近的其他研究（Dobbins和Kuijt，1973年; Musselman和Dickison，1975年; Weber，1976年a）是基于从野外收集的材料。
The initiation and early developmental stages of the haustorium were studied in Agalinis purpurea (Scrophulariaceae). Plants were grown in a O.9Wo agar inorganic medium with a 0.5% sucrose supplement. Root exudate collected from Lespedeza sericea induced the initiation of haustoria, with earliest stages evident in 6-12 hr. A 30-min exposure to exudate produced an increased frequency of haustoria and a 24-hr exposure yielded haustorial frequencies equal to the number that were initiated on control plants continuously exposed to root exudate for the 5-day growth period. The early cytological features of haustorial development are described and the possible significance of haustorial initiation in host recognition is discussed. AGALINIS PURPUREA (L.) Raf. is a chlorophyll-containing root parasite of the Scrophulariaceae. This plant is a widespread herbaceous annual of eastern North America. Like other hemiparasites in the subfamily Rhinanthoideae, it produces specialized root structures termed haustoria which form a morphological and physiological bridge between host and parasite. Haustorial structure has been described in numerous studies although the process of initiation and development has received little attention (Kuijt, 1977). The only experimental work on haustorial development has been that of Malcolm (1966) on Castilleja coccinea and Melampyrum lineare, Thurman (1966) on several species of Orthocarpus, and Atsatt (1973) who reported on agar cultures of Orthocarpus purpurascens. These studies did not focus on the cytological details of initiation. Developmental observations of early parasite contact by Striga asiatica (Rogers and Nelson, 1962) and with Alectra vogelii (Visser, Dorr, and Kollmann, 1977) concerned direct transformation of the root apex into a haustorium upon host contact. Other recent studies (Dobbins and Kuijt, 1973; Musselman and Dickison, 1975; Weber 1976a) are based on collections from field material.