Whole-mount Confocal Microscopy for Adult Ear Skin: A Model System to Study Neuro-vascular Branching Morphogenesis and Immune Cell Distribution
Whole-mount Confocal Microscopy for Adult Ear Skin: A Model System to Study Neuro-vascular Branching Morphogenesis and Immune Cell Distribution
复制标题
DOI：
10.3791/57406
复制
发表时间：
2018-03-01
影响因子：
1.2
通讯作者：
Mukouyama, Yoh-Suke
中科院分区：
文献类型：
--
作者：
Yamazaki, Tomoko;Li, Wenling;Mukouyama, Yoh-Suke
在这里，我们提出了一种完整的成人耳部皮肤成像技术方案，以研究全面的三维神经血管分支形态发生和模式，以及细胞水平上的免疫细胞分布。对成人组织中周围神经和血管解剖结构的分析，为理解创伤愈合等病理状态下的神经血管连接和神经血管变性提供了一些见解。作为一个高信息量的模型系统，我们的研究重点是成人耳皮肤，它很容易被解剖。我们的简单和可重复的方案提供了整个皮肤细胞成分的准确描述，如周围神经（感觉轴突、交感轴突和雪旺细胞）、血管（内皮细胞和血管平滑肌细胞）和炎症细胞。我们相信该方案将为研究不同病理条件下成人耳皮肤周围神经和血管的形态学异常以及炎症的发生铺平道路。
Here, we present a protocol of a whole-mount adult ear skin imaging technique to study comprehensive three-dimensional neuro-vascular branching morphogenesis and patterning, as well as immune cell distribution at a cellular level. The analysis of peripheral nerve and blood vessel anatomical structures in adult tissues provides some insights into the understanding of functional neuro-vascular wiring and neuro-vascular degeneration in pathological conditions such as wound healing. As a highly informative model system, we have focused our studies on adult ear skin, which is readily accessible for dissection. Our simple and reproducible protocol provides an accurate depiction of the cellular components in the entire skin, such as peripheral nerves (sensory axons, sympathetic axons, and Schwann cells), blood vessels (endothelial cells and vascular smooth muscle cells), and inflammatory cells. We believe this protocol will pave the way to investigate morphological abnormalities in peripheral nerves and blood vessels as well as the inflammation in the adult ear skin under different pathological conditions.