In vivo molecular contrast OCT imaging of methylene blue.
In vivo molecular contrast OCT imaging of methylene blue.
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DOI：
10.1364/ol.40.001426
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发表时间：
2015-04-01
期刊：
影响因子：
3.6
通讯作者：
Applegate BE
中科院分区：
文献类型：
--
作者：
Kim W;Applegate BE
An 830 nm spectral-domain Optical Coherence Tomography (OCT) system with an integrated 663 nm diode pump laser has been developed to enable molecular contrast OCT imaging of Methylene Blue (MB), a common vital dye used clinically. The introduction of the 663 nm diode laser, which acts as the pump in this implementation of Pump-Probe OCT (PPOCT), represents a minor modification to an otherwise typical OCT system. A newly developed background subtraction technique completely removes all background from intensity noise at the pump modulation frequency, simplifying the interpretation of PPOCT images. These developments have enabled the first in vivo imaging of MB with PPOCT. Volumetric images of a zebrafish, stained by submersion in a 0.01% (w/v) solution of MB for 6 hours, show accumulation of MB in the mesonephros, the primordial filtration organ.
