In Azospirillum brasilense, mutations in flmA or flmB genes affect polar flagellum assembly, surface polysaccharides, and attachment to maize roots
In Azospirillum brasilense, mutations in flmA or flmB genes affect polar flagellum assembly, surface polysaccharides, and attachment to maize roots
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DOI：
10.1016/j.micres.2016.05.006
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发表时间：
2016-01-01
影响因子：
6.7
通讯作者：
Jofre, Edgardo
中科院分区：
文献类型：
--
作者：
Ariel Rossi, Fernando;Beatriz Medeot, Daniela;Jofre, Edgardo
巴西固氮菌是一种能够促进植物生长的土壤细菌。以前曾报道过几种表面成分参与了A. brasilense到根植物。这些成分包括胞外多糖（EPS）、脂多糖（LPS）和极鞭毛。来自极鞭毛的鞭毛蛋白是糖基化的，并且有人认为参与这种翻译后修饰的基因与参与LPS的O-抗原中存在的糖的生物合成的基因相同。在这项工作中，我们报告的两个同源物的特性存在于A。brasilense Cd的鞭毛蛋白修饰基因flmA和flmB。我们发现，无论是flmA或flmB基因突变的A。brasilense由于极鞭毛组装的改变而导致非运动细胞。此外，这些突变也影响了A. brasilense细胞吸附到玉米根上并产生LPS和EPS。通过产生一个突变体含有极鞭毛的影响，在他们的旋转，我们显示的重要性，细菌的运动性的早期殖民化的玉米根。(C)2016 Elsevier GmbH. All rights reserved.
Azospirillum brasilense is a soil bacterium capable of promoting plant growth. Several surface components were previously reported to be involved in the attachment of A. brasilense to root plants. Among these components are the exopolysaccharide (EPS), lipopolysaccharide (LPS) and the polar flagellum. Flagellin from polar flagellum is glycosylated and it was suggested that genes involved in such a posttranslational modification are the same ones involved in the biosynthesis of sugars present in the O-antigen of the LPS. In this work, we report on the characterization of two homologs present in A. brasilense Cd, to the well characterized flagellin modification genes, flmA and flmB, from Aeromonas caviae. We show that mutations in either flmA or flmB genes of A. brasilense resulted in non-motile cells due to alterations in the polar flagellum assembly. Moreover, these mutations also affected the capability of A. brasilense cells to adsorb to maize roots and to produce LPS and EPS. By generating a mutant containing the polar flagellum affected in their rotation, we show the importance of the bacterial motility for the early colonization of maize roots. (C) 2016 Elsevier GmbH. All rights reserved.