Caveolin‐1 expression in Schwann cells-猫眼课题宝

Caveolin-1 在雪旺细胞中的表达

DOI：

发表时间：

1999

期刊：

Glia

影响因子：

6.2

通讯作者：

中科院分区：

医学1区

文献类型：

作者：

D. Mikol;Hoylond L. Hong;Hsin‐Lin Cheng;E. Feldman

小窝是质膜的非网格蛋白包被的内陷，其存在于大多数细胞类型中。小窝的一个组成部分是相关蛋白的小窝蛋白家族，其不仅形成小窝的结构框架，而且可能维持其功能作用，包括调节信号转导和细胞转运，特别是胆固醇运输。虽然小窝已被确定在周围神经系统（PNS）的超微结构，小窝蛋白的表达以前没有被研究。迄今为止，已经报道了三个小窝蛋白基因。在这里，我们首次表明小窝蛋白-1由雪旺细胞（SC）以及几种SC衍生的细胞系表达。小窝蛋白-1富含于大鼠坐骨神经（SN）和SC的浮力、洗涤剂不溶性膜中，这是小窝区室的标志。小窝蛋白-1在大鼠SN和SC中以可溶性和不溶性形式存在，并定位于SC细胞质和轴突髓鞘。当SC恢复到髓鞘形成前的表型时，SC caveolin-1在轴突切断后减少。我们推测小窝蛋白-1可以调节髓鞘形成SC中的信号转导和/或胆固醇转运。GLIA 27：39-52，1999。© 1999 Wiley‐利斯公司

Caveolae are non‐clathrin‐coated invaginations of the plasma membrane, which are present in most cell types. An integral component of caveolae is the caveolin family of related proteins, which not only forms the structural framework of caveolae, but also likely subserves its functional roles, including regulation of signal transduction and cellular transport, in particular, cholesterol trafficking. Although caveolae have been identified ultrastructurally in the peripheral nervous system (PNS), caveolin expression has not previously been studied. To date, three caveolin genes have been reported. Here, we show for the first time that caveolin‐1 is expressed by Schwann cells (SC) as well as several SC‐derived cell lines. Caveolin‐1 is enriched in the buoyant, detergent‐insoluble membranes of rat sciatic nerve (SN) and SC, a hallmark of the caveolar compartment. Caveolin‐1 exists as both soluble and insoluble forms in rat SN and SC, and localizes to SC cytoplasm and abaxonal myelin. SC caveolin‐1 decreases after axotomy, when SC revert to a premyelinating phenotype. We speculate that caveolin‐1 may regulate signal transduction and/or cholesterol transport in myelinating SC. GLIA 27:39–52, 1999. © 1999 Wiley‐Liss, Inc.