A common idiotope on human rheumatoid factors identified by a hybridoma antibody.
A common idiotope on human rheumatoid factors identified by a hybridoma antibody.
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由杂交瘤抗体鉴定出的人类类风湿因子的常见独特位。
DOI：
10.1016/0161-5890(83)90116-5
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发表时间：
1983
影响因子：
3.6
通讯作者：
Fong,S
中科院分区：
文献类型：
--
作者：
Carson,DA;Fong,S
人单克隆和多克隆抗igg自身抗体[类风湿因子（RFs）]主要由kappa轻链组成，并可能表现出交叉反应独特型。然而，共享特性的性质仍然不清楚。我们制备了一种小鼠杂交瘤抗体（17-109），该抗体识别30%（310）的人IgM-RF副蛋白上存在的独特位体，而在没有RF活性的免疫球蛋白上不存在。在分离的、完整的kappa轻链上可以测量独特型，但在轻链色氨酸和分离的重链上不能测量独特型。比较了5种已知kappa链氨基酸序列的IgM-RF副蛋白与17-109的结合，表明杂交瘤识别的独特型与互补决定区之间存在关系。类风湿关节炎患者的血清中含有独特型阳性物质，特异地与17-109免疫吸附柱结合。17-109抗独特位抗体部分抑制血清IgM- rf和IgA- rf与IgG的结合，但不影响IgM和IgA抗破伤风类毒素抗体与抗原的结合。这些结果表明，相当大比例的IgM-RF副蛋白共享位于kappa轻链互补决定区域或附近的独特体。人血清rf包括一个kappa轻链家族，它与IgM-RF副蛋白上的kappa链具有独特的相关性。
Human monoclonal and polyclonal anti-IgG autoantibodies [rheumatoid factors (RFs)] are composed primarily of kappa light chains, and may display cross-reactive idiotypes. However, the nature of the shared idiotope (s) has remained unclear. We have prepared a murine hybridoma antibody (17–109) that recognizes an idiotope present on 30%(3 10) of human IgM-RF paraproteins, and absent on immunoglobulins without RF activity. The idiotope was measurable on isolated, intact kappa light chains, but not on light-chain tryptic peptides, nor on isolated heavy chains. A comparison of the binding to 17–109 of five IgM-RF paraproteins, with known kappa chain amino acid sequences, suggested a relationship between the idiotope recognized by the hybridoma and the complementarity-determining regions. The serum of patients with rheumatoid arthritis contained idiotope positive material that bound specifically to a 17–109 immunoadsorbent column. Moreover, the 17–109 anti-idiotope antibody partially inhibited the binding to IgG of IgM-RF and IgA-RF in serum, but did not effect the binding to antigen of IgM and IgA anti-tetanus toxoid antibodies. These results suggest that a significant proportion of IgM-RF paraproteins share an idiotope located at or near the complementarity-determining regions of the kappa light chain. Human serum RFs include a kappa light chain family that is idiotopically related to the kappa chains on IgM-RF paraproteins.