Expression pattern of embryonic stem cell markers in DFAT cells and ADSCs
Expression pattern of embryonic stem cell markers in DFAT cells and ADSCs
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DOI：
10.1007/s11033-011-1371-4
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发表时间：
2012-05-01
影响因子：
2.8
通讯作者：
Yang, Gongshe
中科院分区：
文献类型：
--
作者：
Gao, Qian;Zhao, Lili;Yang, Gongshe
成熟脂肪细胞在天花板法条件下可以恢复为更原始的表型并获得细胞增殖能力，称为去分化脂肪细胞（DFAT cells）。这些细胞表现出作为脂肪组织来源的基质细胞（ADSC）的多谱系潜能。然而，DFAT细胞的干细胞分子特征以及与ADSC的区别仍然不确定。为了更好地研究DFAT细胞的分子特征，从大鼠体内获得了高纯度的成熟脂肪细胞，纯度大于98%，其中约98.6%为单核细胞。这些成熟的脂肪细胞通过天花板培养法自发地去分化为成纤维细胞样细胞，这些细胞在体外快速增殖，沿同一方向生长并形成顶点，并广泛表达胚胎干细胞标志物如Oct 4、Sox 2、c-Myc和Nanog，表面抗原SSEA-1、CD 105和CD 31，并且这些细胞具有ALP和端粒酶活性。DFAT细胞的表达水平分别为Oct 4 1.3%、Sox 2 1.3%、c-Myc 1.2%、Nanog 1.2%、CD 105 0.6%、CD 31 0.6%和SSEA-1 0.4%，均低于脂肪干细胞，但DFAT细胞的纯度远高于脂肪干细胞。结论：DFAT细胞是一个高纯度的干细胞群体，表达ADSCs等胚胎干细胞标志物，是未来细胞替代治疗的一个很好的成体干细胞候选来源。
Mature adipocytes can revert to a more primitive phenotype and gain cell proliferative ability under the condition of ceiling method, named dedifferentiated fat cells (DFAT cells). These cells exhibit multilineage potential as adipose tissue-derived stromal cells (ADSCs). However, the stem molecular signature of DFAT cells and the difference distinct from ADSCs are still not sure. To study the molecular signature of DFAT cells better, highly purified mature adipocytes were obtained from rats and the purity was more than 98%, and about 98.6% were monocytes. These mature adipocytes dedifferentiated into fibroblast-like cells spontaneously by the ceiling culture method, these cells proliferated rapidly in vitro, grew in the same direction and formed vertex, and expressed extensively embryonic stem cell markers such as Oct4, Sox2, c-Myc, and Nanog, surface antigen SSEA-1, CD105, and CD31, moreover, these cells possessed ALP and telomerase activity. The expression level was Oct4 1.3%, Sox2 1.3%, c-Myc 1.2%, Nanog 1.2%, CD105 0.6%, CD31 0.6% and SSEA-1 0.4%, respectively, which was lower than that in ADSCs, but the purity of DFAT cells was much higher than that of ADSCs. In conclusion, DFAT cells is a highly purified stem cell population, and expressed some embryonic stem cell markers like ADSCs, which seems to be a good candidate source of adult stem cells for the future cell replacement therapy.