DMSO regulates osteoclast development in vitro.
DMSO regulates osteoclast development in vitro.
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DMSO 在体外调节破骨细胞发育。
DOI：
10.1007/s11626-011-9385-8
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Kacena,MelissaA
中科院分区：
文献类型：
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作者：
Lemieux,JustinM;Wu,Gary;Morgan,JosephA;Kacena,MelissaA
二甲基亚砜（DMSO）在实验室中通常用作有机分子的溶剂和载体。虽然它已被用于以前的研究涉及骨髓细胞和巨噬细胞，我们不知道的数据表明，DMSO单独对破骨细胞的发展的影响。最近，我们使用DMSO作为溶剂，并包括非溶剂对照。令人惊讶的是，我们观察到破骨细胞发育的显着变化，因此设计了这项研究，以检查DMSO对破骨细胞发育的影响。破骨细胞由两种来源产生：骨髓巨噬细胞和破骨细胞祖细胞系。将细胞用DMSO以不同的浓度培养不同的持续时间，并在细胞数量、细胞表面积和细胞核数量/细胞方面评估成熟的多核（>3个核）TRAP+细胞。破骨细胞表面积在5 μM DMSO中从对照培养物中的平均38，510像素增加至平均156，422像素，随后在10 μM DMSO中降低至平均18，994像素。随着连续添加DMSO超过5天，平均表面积的显着增加，和细胞核/细胞的数量也观察到，而相反的是真实的，当DMSO连续从培养物中移除。这些结果表明，DMSO对破骨细胞分化有明显的影响。由于许多研究者使用DMSO溶解化合物以治疗破骨细胞，因此需要谨慎，因为改变DMSO浓度可能对最终数据产生深远影响，特别是在评估破骨细胞分化的情况下。
Dimethyl sulfoxide (DMSO) is routinely used in the laboratory as a solvent and vehicle for organic molecules. Although it has been used in previous studies involving myeloid cells and macrophages, we are unaware of data demonstrating the effects of DMSO alone on osteoclast development. Recently, we were using DMSO as a vehicle and included a non-vehicle control. Surprisingly, we observed a marked change in osteoclast development, and therefore designed this study to examine the effects of DMSO on osteoclast development. Osteoclasts were generated from two sources: bone marrow macrophages and an osteoclast progenitor cell line. Cells were cultured with DMSO for various durations and at differing concentrations and mature, multinucleated (>3 nuclei) TRAP+cells were assessed in terms of cell number, cell surface area, and number of nuclei/cell. Osteoclast surface area increased in 5 μM DMSO to a mean of 156,422 pixels from a mean of 38,510 pixels in control culture, and subsequently decreased in 10 μM DMSO to a mean of 18,994 pixels. With serial addition of DMSO over 5 d, a significant increase in mean surface area, and number of nuclei/cell was also observed, while the opposite was true when DMSO was serially removed from culture. These findings show that DMSO exerts a marked effect on osteoclast differentiation. Since many investigators use DMSO to solubilize compounds for treatment of osteoclasts, caution is warranted as altering DMSO concentrations may have a profound effect on the final data, especially if osteoclast differentiation is being assessed.