LncRNA SNHG15 acts as an oncogene in prostate cancer by regulating miR-338-3p/FKBP1A axis
LncRNA SNHG15 acts as an oncogene in prostate cancer by regulating miR-338-3p/FKBP1A axis
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DOI：
10.1016/j.gene.2019.04.033
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发表时间：
2019-07-15
期刊：
影响因子：
3.5
通讯作者：
Zhang, Qi
中科院分区：
文献类型：
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作者：
Zhang, Yuelong;Zhang, Dahong;Zhang, Qi
长链非编码RNA（lncRNA）是多种疾病进展的重要调控因子。尽管近年来人们对lncRNA在前列腺癌（PCa）中的作用进行了研究，但仍有大量的lncRNA需要阐明。本研究旨在探讨lncRNA小核仁RNA宿主基因15（SNHG 15）在人前列腺癌中的作用。使用qRT-PCR分析，我们鉴定了PCa细胞系中SNHG 15的上调。进行功能丧失测定以确定SNHG 15对PCa细胞增殖、迁移和上皮-间充质转化（EMT）的调节作用。功能检测结果表明，SNHG 15基因敲低后，细胞活力降低，增殖抑制，迁移和侵袭能力降低，EMT进程逆转。这些结果揭示了SNHG 15在PCa中的致癌功能。SNHG 15定位于PCa细胞的胞浆中，是microRNA-338- 3 p（miR-338- 3 p）的分子海绵。此外，FKBP脯氨酰异构酶1A（FKBP 1A）是miR-338- 3 p的靶点。这项研究表明，SNHG 15可能作为竞争性内源性RNA（ceRNA）调节miR-338- 3 p和FKBP 1A。最后，通过进行拯救测定证明miR-338- 3 p和FKBP 1A参与SNHG 15介导的生物学功能。总之，我们的研究揭示了一个新的途径在PCa中的功能。
Long non-coding RNAs (lncRNAs) are crucial regulators in the progression of various diseases. Although the role of lncRNAs in prostate cancer (PCa) has been studied in recent years, there are still numerous lncRNAs need to be elucidated. This study aims to detect the role of lncRNA small nucleolar RNA host gene 15 (SNHG15) in human prostate cancer. Using qRT-PCR analysis, we identified the upregulation of SNHG15 in PCa cell lines. Loss-of function assays were conducted to determine the regulatory effect of SNHG15 on PCa cell proliferation, migration and epithelial-mesenchymal transition (EMT). According to the results of functional assays, we found that knockdown of SNHG15 impaired cell viability, suppressed cell proliferation, inhibited cell migration and invasion, reversed EMT progress. All these findings revealed the oncogenic function of SNHG15 in PCa. Mechanism investigation revealed that SNHG15 was located in the cytoplasm of PCa cells and acted as a molecular sponge of microRNA-338-3p (miR-338-3p). Moreover, FKBP prolyl isomerase 1A (FKBP1A) was a target of miR-338-3p. This investigation demonstrated that SNHG15 may serve as a competing endogenous RNA (ceRNA) to regulate miR-338-3p and FKBP1A. Finally, the involvement of miR-338-3p and FKBP1A in SNHG15-mediated biological function was demonstrated by performing rescue assays. In summary, our study revealed the function of a novel pathway in PCa.