Immunohistochemical analysis of subcutaneous tissue reactions to methacrylate resin-based root canal sealers
Immunohistochemical analysis of subcutaneous tissue reactions to methacrylate resin-based root canal sealers
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DOI：
10.1111/j.1365-2591.2011.01874.x
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发表时间：
2011-07-01
影响因子：
5
通讯作者：
Okiji, T.
中科院分区：
文献类型：
--
作者：
Yamanaka, Y.;Shigetani, Y.;Okiji, T.
目的通过免疫组化评价炎症/免疫活性细胞浸润，探讨甲基丙烯酸酯树脂基根管密封剂对皮下组织的反应。方法将含有新配制的主显灵SE密封剂、MetaSEAL、Super-Bond RC密封剂或氧化锌-丁香酚密封剂（Canals）的硅胶管皮下植入Wistar大鼠背部。植入不同动物体内的固体硅棒作为对照。7、14和28天后，对植入物周围的结缔组织（每个n = 8）进行处理，使用OX6（对主要组织相容性复合体II类分子反应）、ED1（对巨噬细胞反应）和W3/13（主要对中性粒细胞反应）进行免疫过氧化物酶染色，并计算每个区（1.2 x 0.8 mm）内阳性染色细胞的数量。统计差异分析采用Friedman检验和Scheffe检验（检验材料之间的比较）或Mann-Whitney u检验（检验-对照比较）。结果28 d时w3 /13阳性细胞数（以中性粒细胞为主）明显高于MetaSEAL （P < 0.05）。各测试材料在任何时间点的OX6-或ed1阳性细胞数量均无显著差异。测试-对照比较揭示了每种抗体的几个显著差异。这在ED1中最为显著，每个时间点的所有测试材料，除了28天的主显节SE，都显示出明显大于相应对照的值。结论所有的甲基丙烯酸酯树脂基密封剂均有相似水平的炎症/免疫活性细胞浸润。MetaSEAL诱导的中性粒细胞浸润强度低于Canals。与所有测试材料相比，对照组的炎症/免疫能力细胞浸润较轻。
P>AimTo investigate subcutaneous tissue reactions to methacrylate resin-based root canal sealers by immunohistochemical assessment of inflammatory/immunocompetent cell infiltration.MethodologySilicone tubes containing freshly mixed Epiphany SE sealer, MetaSEAL, Super-Bond RC sealer, or a zinc oxide-eugenol sealer (Canals) were subcutaneously implanted into the backs of Wistar rats. Solid silicone rods implanted in different animals served as controls. After 7, 14 and 28 days, connective tissue surrounding the implants (n = 8, each) was processed for immunoperoxidase staining using OX6 (reactive to major histocompatibility complex class II molecules), ED1 (reactive to macrophages), and W3/13 (reactive primarily to neutrophils), and the number of positively stained cells within each field (1.2 x 0.8 mm) was enumerated. Statistical differences were analysed with Friedman's test and Scheffe's test (comparisons between test materials) or Mann-Whitney's U-test (test-control comparisons).ResultsCanals showed a significantly higher number of W3/13-positive cells (mostly neutrophils) than MetaSEAL at 28 days (P < 0.05). There were no significant differences in the numbers of OX6- or ED1-positive cells between each test material at any time point. Test-control comparisons revealed several significant differences for each antibody. This was most notable for ED1, where all the test materials at each time point, except for Epiphany SE at 28 days, showed significantly larger values than the corresponding controls.ConclusionsAll the methacrylate resin-based sealers tested showed a similar level of inflammatory/immunocompetent cell infiltration. MetaSEAL induced less-intense neutrophil infiltration than Canals. Controls exhibited milder infiltration of inflammatory/immunocompetent cells compared with all the test materials.