Radiation damage in protein serial femtosecond crystallography using an x-ray free-electron laser.
Radiation damage in protein serial femtosecond crystallography using an x-ray free-electron laser.
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DOI：
10.1103/physrevb.84.214111
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发表时间：
2011-12-01
期刊：
影响因子：
--
通讯作者：
Schlichting I
中科院分区：
文献类型：
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作者：
Lomb L;Barends TR;Kassemeyer S;Aquila A;Epp SW;Erk B;Foucar L;Hartmann R;Rudek B;Rolles D;Rudenko A;Shoeman RL;Andreasson J;Bajt S;Barthelmess M;Barty A;Bogan MJ;Bostedt C;Bozek JD;Caleman C;Coffee R;Coppola N;Deponte DP;Doak RB;Ekeberg T;Fleckenstein H;Fromme P;Gebhardt M;Graafsma H;Gumprecht L;Hampton CY;Hartmann A;Hauser G;Hirsemann H;Holl P;Holton JM;Hunter MS;Kabsch W;Kimmel N;Kirian RA;Liang M;Maia FR;Meinhart A;Marchesini S;Martin AV;Nass K;Reich C;Schulz J;Seibert MM;Sierra R;Soltau H;Spence JC;Steinbrener J;Stellato F;Stern S;Timneanu N;Wang X;Weidenspointner G;Weierstall U;White TA;Wunderer C;Chapman HN;Ullrich J;Strüder L;Schlichting I
X射线自由电子激光器提供强烈的飞秒脉冲，有望在样品被辐射损伤破坏之前产生纳米晶体的高分辨率衍射数据。使用2keV光子在直线加速器相干光源处收集的溶菌酶纳米晶体的衍射强度用于通过分子置换的结构测定，并分析作为脉冲长度和能量密度的函数的辐射损伤。辐射损伤的签名观察到的脉冲短至70 fs。常规晶体学中使用的参数标度不能解释观察到的效应。
X-ray free-electron lasers deliver intense femtosecond pulses that promise to yield high resolution diffraction data of nanocrystals before the destruction of the sample by radiation damage. Diffraction intensities of lysozyme nanocrystals collected at the Linac Coherent Light Source using 2 keV photons were used for structure determination by molecular replacement and analyzed for radiation damage as a function of pulse length and fluence. Signatures of radiation damage are observed for pulses as short as 70 fs. Parametric scaling used in conventional crystallography does not account for the observed effects.