Fackel interacts with gibberellic acid signaling and vernalization to mediate flowering in Arabidopsis
Fackel interacts with gibberellic acid signaling and vernalization to mediate flowering in Arabidopsis
复制标题
Fackel 与赤霉酸信号传导和春化作用相互作用以介导拟南芥开花
DOI：
10.1007/s00425-017-2652-5
复制
发表时间：
2017-05-01
期刊：
影响因子：
4.3
通讯作者：
Hou, Suiwen
中科院分区：
文献类型：
--
作者：
Huang, Bingyao;Qian, Pingping;Hou, Suiwen
Fackel （FK）主要通过赤霉素途径和春化途径参与拟南芥的开花过程。这一新功能部分依赖于开花位点C（FLC）。高等植物在其生命周期中存在着从营养阶段到生殖阶段的共同过渡过程。花芽分化的发生在植物生殖阶段起着关键作用，受到外部环境和内部调控因素的双重影响。在本研究中，我们发现编码参与固醇生物合成的C-14还原酶的FACKEL （FK）基因受损的拟南芥弱突变等位基因FK - j3158在不同光周期下具有较长的生命周期和较晚的开花时间。此外，FK过表达系的开花表型比野生型早。这些过程可能独立于油菜素内酯（BR）下游途径和自主途径。赤霉素（GA）处理下，fk-J3158植株抽苔天数和总叶数的减少比野生型更为敏感。进一步研究表明，FK突变导致FK - j3158中内源性GAs缺失，GA和多效唑（paclobutrazol， PAC）处理也影响了FK基因的表达。此外，通过3周春化处理，fk-J3158的延迟开花时间得以恢复，开花位点C （FLC）的表达也相应下调。在长日照（LD）条件下，fk-J3158 flc双突变体的开花时间明显早于fk-J3158。这些结果表明，FK可能主要通过GA途径和春化途径影响拟南芥的开花。这些效应部分依赖于开花位点C （FLC）。
Fackel (FK) is involved in the flowering ofArabidopsismainly via the gibberellin pathway and vernalization pathway. This new function of FK is partially dependent on theFLOWERING LOCUS C(FLC). A common transitional process from vegetative stage to reproductive stage exists in higher plants during their life cycle. The initiation of flower bud differentiation, which plays a key role in the reproductive phase, is affected by both external environmental and internal regulatory factors. In this study, we showed that the Arabidopsis weak mutant allele fk-J3158, impaired in the FACKEL (FK) gene, which encodes a C-14 reductase involved in sterol biosynthesis, had a long life cycle and delayed flowering time in different photoperiods. In addition, FK overexpression lines displayed an earlier flowering phenotype than that of the wild type. These processes might be independent of the downstream brassinosteroid (BR) pathway and the autonomous pathway. However, the fk-J3158 plants were more sensitive than wild type in reducing the bolting days and total leaf number under gibberellic acid (GA) treatment. Further studies suggested that FK mutation led to an absence of endogenous GAs in fk-J3158 and FK gene expression was also affected under GA and paclobutrazol (PAC) treatment. Moreover, the delayed flowering time of fk-J3158 could be rescued by a 3-week vernalization treatment, and the expression of FLOWERING LOCUS C (FLC) was accordingly down-regulated in fk-J3158. We also demonstrated that flowering time of fk-J3158 flc double mutant was significantly earlier than that of fk-J3158 under the long-day (LD) conditions. All these results indicated that FK may affect the flowering in Arabidopsis mainly via GA pathway and vernalization pathway. And these effects are partially dependent on the FLOWERING LOCUS C (FLC).