Array-based comparative genomic hybridization for the differential diagnosis of renal cell cancer.
Array-based comparative genomic hybridization for the differential diagnosis of renal cell cancer.
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发表时间：
2002-02
期刊：
影响因子：
11.2
通讯作者：
M. Wilhelm;J. Veltman;A. Olshen;Ajay N. Jain;D. Moore;J. Presti;G. Kovacs;F. Waldman
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文献类型：
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作者：
M. Wilhelm;J. Veltman;A. Olshen;Ajay N. Jain;D. Moore;J. Presti;G. Kovacs;F. Waldman
基于阵列的比较基因组杂交 (CGH) 使用排列在载玻片上的多个基因组克隆来检测肿瘤 DNA 序列的相对拷贝数。将阵列 CGH 应用于肿瘤标本使得癌症的基因诊断成为可能，并可能有助于在生物学和临床上区分相关的肿瘤亚群，从而做出更好的预后和治疗决策。在这项研究中，我们使用基于阵列的 CGH 来检测不同类型肾细胞癌中 DNA 拷贝数的变化，以用于诊断目的。我们能够通过自动计算手段正确诊断 34 种恶性肿瘤中的 33 种，并将 8 种良性肿瘤和正常肾脏样本归为一组。这些结果表明基于阵列的 CGH 能够根据基因图谱诊断绝大多数肾细胞癌。
Array-based comparative genomic hybridization (CGH) uses multiple genomic clones arrayed on a slide to detect relative copy number of tumor DNA sequences. Application of array CGH to tumor specimens makes genetic diagnosis of cancers possible and may help to differentiate relevant subsets of tumors, biologically and clinically, which would allow better prognostic and therapeutic decision making. In this study, we have used array-based CGH to detect DNA copy number alterations in distinct types of renal cell carcinomas for diagnostic purposes. We were able to correctly diagnose 33 of 34 malignant tumors by automated computational means and to group together eight benign neoplasms and normal kidney samples. These results indicate that array-based CGH is capable of diagnosing the vast majority of renal cell carcinomas based on their genetic profiles.