UVB Inhibits Proliferation, Cell Cycle and Induces Apoptosis via p53, E2F1 and Microtubules System in Cervical Cancer Cell Lines.
UVB Inhibits Proliferation, Cell Cycle and Induces Apoptosis via p53, E2F1 and Microtubules System in Cervical Cancer Cell Lines.
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DOI：
10.3390/ijms22105197
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发表时间：
2021-05-14
影响因子：
5.6
通讯作者：
López JA
中科院分区：
文献类型：
--
作者：
Granados-López AJ;Manzanares-Acuña E;López-Hernández Y;Castañeda-Delgado JE;Fraire-Soto I;Reyes-Estrada CA;Gutiérrez-Hernández R;López JA
紫外线（UV）暴露与皮肤损伤和致癌有关，但最近UVB已被提议作为癌症的治疗方法。在此，我们研究了UVB在永生和致瘤HPV阳性和阴性细胞中的细胞和分子效应。用220.5 ~ 1102.5J/m2的UVB照射细胞，结晶紫法检测细胞增殖，流式细胞仪检测细胞周期阻滞和凋亡。UVB对细胞的影响在661.5 J/m2时记录，在1102.5 J/m2时加剧。所有细胞系均受到增殖抑制、细胞周期阻断和凋亡诱导的影响，其程度取决于成瘤水平或HPV类型。众所周知的UV响应p53，E2 F1和微管系统蛋白的分析在SiHa细胞中进行响应UVB通过蛋白质印迹分析。E2 F1和微管相关蛋白2（MAP 2）表达下降与细胞过程改变相关，而p53和微管相关蛋白1 S（MAP 1 S）表达开关自882 J/m2起观察到，这表明它们在更严重的细胞损伤下是必需的。然而，α-Tubulin 3C和β-Tubulin的表达转变突然被注意到，直到1102.5 J/m2，特别是γ-Tubulin蛋白表达保持不变。这项研究提供了深入了解UVB在宫颈癌细胞系中的作用。
Ultraviolet (UV) exposure has been linked to skin damage and carcinogenesis, but recently UVB has been proposed as a therapeutic approach for cancer. Herein, we investigated the cellular and molecular effects of UVB in immortal and tumorigenic HPV positive and negative cells. Cells were irradiated with 220.5 to 1102.5 J/m2 of UVB and cell proliferation was evaluated by crystal violet, while cell cycle arrest and apoptosis analysis were performed through flow cytometry. UVB effect on cells was recorded at 661.5 J/m2 and it was exacerbated at 1102.5 J/m2. All cell lines were affected by proliferation inhibition, cell cycle ablation and apoptosis induction, with different degrees depending on tumorigenesis level or HPV type. Analysis of the well-known UV-responsive p53, E2F1 and microtubules system proteins was performed in SiHa cells in response to UVB through Western-blotting assays. E2F1 and the Microtubule-associated protein 2 (MAP2) expression decrease correlated with cellular processes alteration while p53 and Microtubule-associated Protein 1S (MAP1S) expression switch was observed since 882 J/m2, suggesting they were required under more severe cellular damage. However, expression transition of α-Tubulin3C and β-Tubulin was abruptly noticed until 1102.5 J/m2 and particularly, γ-Tubulin protein expression remained without alteration. This study provides insights into the effect of UVB in cervical cancer cell lines.
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DOI：
10.1016/j.bbrc.2007.11.179
发表时间：
2008-02-08
影响因子：
3.1
作者：
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影响因子：
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作者：
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DOI：
10.1074/jbc.m112.357038
发表时间：
2012-05-18
期刊：
The Journal of biological chemistry
影响因子：
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作者：
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通讯作者：
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影响因子：
5.4
作者：
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HOWLEY, PM