8-Carbon oxylipins inhibit germination and growth, and stimulate aerial conidiation in Aspergillus nidulans
8-Carbon oxylipins inhibit germination and growth, and stimulate aerial conidiation in Aspergillus nidulans
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DOI：
10.1016/j.funbio.2011.02.005
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发表时间：
2011-04-01
期刊：
影响因子：
2.5
通讯作者：
Ugalde, Unai
中科院分区：
文献类型：
--
作者：
Herrero-Garcia, Erika;Garzia, Aitor;Ugalde, Unai
当接种量大于1×10~(-5)分生孢子量(-1)时，液体培养物中的孢子萌发逐渐受到抑制。较高的分生孢子密度也会抑制邻近菌丝的生长。在含有3-辛酮和3-辛醇的组分中，8碳氧合脂肪1-辛烯-3-醇是主要的抑制物，这3种氧合脂肪也能提高暗培养表面培养物的分生孢子率，但对液体培养物无影响。3-辛酮是产孢量最大的化合物。3-辛酮的作用需要发育激活剂Flug、FlbB-D和brlA的功能形式，对渗透胁迫或碳饥饿等胁迫刺激的分生孢子效应没有附加作用。氧化脂质在菌丝接触大气后不久产生，对气生菌丝体最有效，表明它们在气相中发挥信号功能。(C)2011年英国真菌学会。爱思唯尔有限公司出版。保留所有权利。
Germination of Aspergillus nidulans conidia in liquid cultures was progressively inhibited at inoculum loads above 1 x 10(5) conidia mL(-1). High conidial densities also inhibited growth of neighbouring mycelia. The eight-carbon oxylipin 1-octen-3-ol was identified as the main inhibitor in a fraction also containing 3-octanone and 3-octanol.These three oxylipins also increased the conidiation rate of dark-grown surface cultures, but had no effect on liquid cultures. 3-octanone was the most conidiogenic compound. The action of 3-octanone required functional forms of developmental activators fluG, flbB-D and brlA, and was not additive to the conidiogenic effect of stress stimuli such as osmotic stress or carbon starvation.Oxylipins were produced shortly after hyphae made contact with the atmosphere and were most effective on aerial mycelia, indicating that they perform their signalling function in the gas phase. (C) 2011 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.