A heterogeneous ouabain-ATPase interaction in mouse brain.
A heterogeneous ouabain-ATPase interaction in mouse brain.
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DOI：
10.1016/0024-3205(78)90654-9
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发表时间：
1978-12
期刊：
影响因子：
6.1
通讯作者：
M. Marks;N. Seeds
中科院分区：
文献类型：
--
作者：
M. Marks;N. Seeds
与小鼠肾脏中的ATPase不同，小鼠肾脏中的ATPase显示出简单的哇巴因相互作用模式，而小鼠大脑中的ATPase与药物显示出不同的相互作用。这种异质性表现在哇巴因对NaKATPase的抑制，哇巴因与脑组织的结合，以及哇巴因对洋地黄毒素与脑结合的抑制。这种非均相相互作用可明显分解为KI(或Kd)为2×10−7M和1×10−4M的两个组分，肾脏的KI(或Kd)约为10−4M。讨论了哇巴因与脑相互作用异质性的可能机制。
Unlike the ATPase in mouse kidney, which shows a simple pattern of ouabain interaction, the ATPase in mouse brain displays a heterogeneous interaction with the drug. Such heterogeniety is evident in the inhibition of NaKATPase by ouabain, in the binding of ouabain to brain tissue, and in the inhibition of digitoxin binding to brain by ouabain. The heterogeneous interaction can apparently be resolved into two components displaying Ki(or Kd) of 2×10−7M and 1×10−4M.The Ki(or Kd) in kidney was approximately 10−4M. Possible mechanisms for the heterogeniety of the ouabain interaction with brain are discussed.