Conserved sequence preference in DNA binding among recombination proteins: an effect of ssDNA secondary structure
Conserved sequence preference in DNA binding among recombination proteins: an effect of ssDNA secondary structure
复制标题
DOI：
10.1093/nar/27.2.596
复制
发表时间：
1999-01-15
影响因子：
14.9
通讯作者：
Dutreix, M
中科院分区：
文献类型：
--
作者：
Biet, E;Sun, JS;Dutreix, M
重复序列已被认为是真核染色体中的重组元件。我们测试了二核苷酸重复序列是否由于其对重组酶的高亲和力而成为重组的优先位点。我们比较了 scRad51、hsRad51 和 ecRecA 蛋白与具有二核苷酸 GT、CA、CT、GA、GC 或 AT 重复序列的寡核苷酸的结合动力学。由于单链 DNA (ssDNA) 中的二级结构充当完全结合的屏障，因此我们测量了这些寡核苷酸是否能够形成稳定的二级结构。我们发现，重组蛋白的优先结合在这三种蛋白中是保守的，并且主要受 ssDNA 二级结构的影响。
Repetitive sequences have been proposed to be recombinogenic elements in eukaryotic chromosomes, We tested whether dinucleotide repeats sequences are preferential sites for recombination because of their high affinity for recombination enzymes. We compared the kinetics of the binding of the scRad51, hsRad51 and ecRecA proteins to oligonucleotides with repeats of dinucleotides GT, CA, CT, GA, GC or AT, Since secondary structures in single-stranded DNA (ssDNA) act as a barrier to complete binding we measured whether these oligonucleotides are able to form stable secondary structures. We show that the preferential binding of recombination proteins is conserved among the three proteins and is influenced mainly by secondary structures in ssDNA.