INTERCHROMOSOMAL RECOMBINATION IN THE EXTREMELY RADIORESISTANT BACTERIUM DEINOCOCCUS RADIODURANS
INTERCHROMOSOMAL RECOMBINATION IN THE EXTREMELY RADIORESISTANT BACTERIUM DEINOCOCCUS RADIODURANS
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DOI：
10.1128/jb.177.19.5495-5505.1995
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发表时间：
1995-10-01
影响因子：
3.2
通讯作者：
MINTON, KW
中科院分区：
文献类型：
--
作者：
DALY, MJ;MINTON, KW
耐辐射球菌和耐辐射球菌属的其他成员对电离辐射和许多其他破坏DNA的物质具有极强的抵抗力。我们最近表明，重组过程参与体内辐照后的质粒间修复。我们现在直接研究在体内固定期（每个细胞四条染色体）辐照下染色体间的重组。暴露于1.75 Mrad（达到37%存活率所需的剂量，使细胞的四条染色体降解为约500个片段）后，我们确定每条染色体可能有多达175个交叉（每个类核700个交叉）进行修复。此外，这些研究表明，在修复过程中发生的许多交叉是非互惠的。
Deinococcus radiodurans and other members of the genus Deinococcus are remarkable for their extreme resistance to ionizing radiation and many other agents that damage DNA. We have recently shown that recombinational processes participate in interplasmidic repair following in vivo irradiation. We now present direct studies on interchromosomal recombination among chromosomes irradiated in vivo during stationary phase (four chromosomes per cell). Following an exposure to 1.75 Mrad (the dose required to achieve a survival of 37%, which degrades the cells' four chromosomes into about 500 fragments), we determined that there may be as many as 175 crossovers per chromosome (700 crossovers per nucleoid) undergoing repair. In addition, these studies suggest that many of the crossovers occurring during repair are nonreciprocal.