Greenbug feeding-induced resistance to sugarcane aphids in sorghum
Greenbug feeding-induced resistance to sugarcane aphids in sorghum
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DOI：
10.3389/fevo.2023.1105725
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发表时间：
2023-02
期刊：
影响因子：
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通讯作者：
Heena Puri;Edith Ikuze;Jessica Ayala;Isabella Rodriguez;R. Kariyat;J. Louis;Sajjan Grover
中科院分区：
文献类型：
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作者：
Heena Puri;Edith Ikuze;Jessica Ayala;Isabella Rodriguez;R. Kariyat;J. Louis;Sajjan Grover
植物受到多种害虫的侵袭，昆虫食草性可以改变植物的防御机制。植物对特定食草动物的防御反应也可能有助于植食性动物在植物上的生长/生存。一种昆虫的取食可以调节植物的防御，这可能会促进或阻碍随后到来的昆虫的定居。然而，人们对顺序食草性对高粱植株的影响知之甚少。在这项研究中，我们证明了一种专门化的甘蔗蚜虫(SCA；Melanaphim sacchari)在高粱上的生长速度比通用的麦长管蚜(Gb；Schizaphim graminum)要快。我们还确定了SCA在高粱上的预侵染如何影响Gb的入侵，反之亦然。我们的连续食草性实验表明，在GB引发的高粱植株上，SCA的繁殖率较低，然而，情况并非如此。为了评价专化型和通用型蚜虫诱导的植物防御能力的差异，我们监测了蚜虫侵染48h后水杨酸(SA)和茉莉酸(JA)标记基因以及类黄酮生物合成途径基因的表达。结果表明，与SCA侵染相比，Gb侵染诱导了SA和JA相关基因以及类黄酮途径基因(DFR、FNR和FNSII)的表达增加。总体而言，我们的结果表明，感染Gb的植物在早期通过植物激素和类黄酮激活了植物的防御系统，阻碍了传入的SCA的定植，并解释了SCA与Gb相比繁殖成功的原因。
Plants are attacked by multiple insect pest species and insect herbivory can alter plant defense mechanisms. The plant defense responses to a specific herbivore may also contribute to the herbivore growth/survival on plants. Feeding by one insect species can modulate the plant defenses, which can either facilitate or hamper the colonization of subsequent incoming insects. However, little is known about the effect of sequential herbivory on sorghum plants. In this study, we demonstrate that a specialist aphid, sugarcane aphid (SCA; Melanaphis sacchari) grows faster on sorghum than a generalist aphid species, greenbug (GB; Schizaphis graminum). We also determined how the pre-infestation of SCA on sorghum affected the invasion of GB and vice-versa. Our sequential herbivory experiments revealed that SCA reproduction was lower on GB-primed sorghum plants, however, the reverse was not true. To assess the differences in plant defenses induced by specialist vs. generalist aphids, we monitored the expression of salicylic acid (SA) and jasmonic acid (JA) marker genes, and flavonoid biosynthetic pathway genes after 48 h of aphid infestation. The results indicated that GB infestation induced higher expression of SA and JA-related genes, and flavonoid pathway genes (DFR, FNR, and FNSII) compared to SCA infestation. Overall, our results suggested that GB-infested plants activate the plant defenses via phytohormones and flavonoids at early time points and hampers the colonization of incoming SCA, as well as explain the reproductive success of SCA compared to GB.