Development and Application of a Chemically Defined Medium for the In Vitro Production of Porcine Embryos
Development and Application of a Chemically Defined Medium for the In Vitro Production of Porcine Embryos
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DOI：
10.1262/jrd.10-196e
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发表时间：
2011-02-01
影响因子：
1.8
通讯作者：
Yoshioka, Koji
中科院分区：
文献类型：
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作者：
Yoshioka, Koji
猪胚胎体外生产（IVP）系统的最新进展，包括体外卵母细胞成熟，受精和胚胎培养，使我们能够产生可存活的胚胎，这些胚胎在移植到受体后可以发育到足月。这项技术目前正应用于配子/胚胎生物学和农业的发展，以及生产克隆猪和转基因猪。用于胚胎IVP的化学成分确定的培养基可用于精确分析物质对配子发生和早期胚胎发生的物理作用，因为它们消除了生物材料中存在的不确定因子，如血清或血清白蛋白。使用化学成分确定的培养基还提高了培养基配方的可靠性，产生了更高的结果再现性，并通过消除可能被病原体污染的蛋白质制剂来确保培养基的生物安全性。因此，它对于研究和商业目的具有一定的优势。我们最近开发了一个定义的猪胚胎IVP系统，使用单一的基础培养基的基础上组成的猪输卵管液。本文讨论了猪IVP胚胎的发育能力和正常性，以及该系统的局限性和进展。
Recent advances in systems for in vitro production (IVP) of porcine embryos, including in vitro oocyte maturation, fertilization and embryo culture, have enabled us to generate viable embryos that can develop to full term after transfer into recipients. This technology is being applied now to developments in gamete/embryo biology and agriculture, as well as in producing cloned and genetically modified pigs. Chemically defined media for IVP of embryos are useful for a precise analysis of the physical action of substances on gametogenesis and early embryogenesis, because they eliminate undefined factors present in biological materials, such as serum or serum albumin. Use of a chemically defined medium also improves the reliability of media formulations, yields a higher reproducibility of results and ensures biosafety of culture media by eliminating protein preparations, which may be contaminated with pathogens. Therefore, it has certain advantages for research and for commercial purposes. We have recently developed a defined IVP system for porcine embryos using a single basic medium based on the composition of porcine oviductal fluid. This paper discusses the developmental ability and normality of porcine IVP embryos, and limitations and advancements in this system.