Local origin and activity-dependent generation of nestin-expressing protoplasmic astrocytes in CA1
Local origin and activity-dependent generation of nestin-expressing protoplasmic astrocytes in CA1
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DOI：
10.1007/s00429-007-0141-5
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发表时间：
2007-07-01
影响因子：
3.1
通讯作者：
Kempermann, Gerd
中科院分区：
文献类型：
--
作者：
Kronenberg, Golo;Wang, Li-Ping;Kempermann, Gerd
由于报告说，前体细胞在成人室管膜下区（SVZ）有助于再生神经元和胶质细胞在CA 1，我们想知道是否也可能存在类似的迁移途径在生理条件下。永久标记SVZ前体细胞与慢病毒载体的绿色荧光蛋白没有显示任何迁移到CA 1在完整的小鼠大脑中的SVZ。然而，在巢蛋白-GFP报告小鼠中，我们发现胼胝体/肺泡区域内的增殖细胞表达巢蛋白和胶质细胞酸性蛋白，类似于成人齿状回邻近神经源性区域的前体细胞。BrdU给药3周内，CA 1中出现BrdU阳性巢蛋白-GFP表达的原生质星形胶质细胞。类似的前体细胞分离的齿状回和SVZ，nestin-GFP表达细胞从胼胝体/肺泡自我更新和多能在体外，而从CA 1分离的细胞没有。CA 1中表达Nestin-GFP的细胞分化为以S100 β表达为特征的有丝分裂后星形胶质细胞。CA 1区未见新生神经元。CA 1区表达nestin-GFP的星形胶质细胞数量随着环境富集而增加。我们的结论是，在CA 1的天体形成的环境条件的影响。然而，SVZ前体细胞并不有助于CA 1的生理细胞可塑性。
Since reports that precursor cells in the adult subventricular zone (SVZ) contribute to regenerative neuro- and gliogenesis in CA1, we wondered whether a similar route of migration might also exist under physiological conditions. Permanent labeling of SVZ precursor cells with a lentiviral vector for green fluorescent protein did not reveal any migration from the SVZ into CA1 in the intact murine brain. However, in a nestin-GFP reporter mouse we found proliferating cells within the corpus callosum/alveus region expressing nestin and glial fibrillary acidic protein similar to precursor cells in the neighboring neurogenic region of the adult dentate gyrus. Within 3 weeks of BrdU administration, BrdU-positive nestin-GFP-expressing protoplasmic astrocytes emerged in CA1. Similar to precursor cells isolated from the dentate gyrus and the SVZ, nestin-GFP- expressing cells from corpus callosum/alveus were self-renewing and multipotent in vitro, whereas cells isolated from CA1 were not. Nestin-GFP-expressing cells in CA1 differentiated into postmitotic astrocytes characterized by S100 beta expression. No new neurons were found in CA1. The number of nestin-GFP-expressing astrocytes in CA1 was increased by environmental enrichment. We conclude that astrogenesis in CA1 is influenced by environmental conditions. However, SVZ precursor cells do not contribute to physiological cellular plasticity in CA1.