Actin-like filaments in bone cells of cultured mouse calvaria as demonstrated by binding to heavy meromyosin
Actin-like filaments in bone cells of cultured mouse calvaria as demonstrated by binding to heavy meromyosin
复制标题
培养小鼠颅骨骨细胞中的肌动蛋白样丝，通过与重肌球蛋白的结合证明
DOI：
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发表时间：
1975
影响因子：
7.8
通讯作者：
Marijke E Holtrop From
中科院分区：
文献类型：
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作者：
Gregory J King;Marijke E Holtrop From
在骨细胞中发现的多种细胞内丝（直径50-70 A）显示出特异性结合HMM。由于这种特性，这些纤维在生物化学上可能与肌肉肌动蛋白相似。在成骨细胞和骨细胞中，这些反应性细丝以平行于质膜的束定向并填充细胞突起。在破骨细胞中，沿细胞膜沿着排列的纤维并不那么整齐。在透明区，细胞的静止部分邻近于活动的皱褶边缘，有组织的细丝束垂直于细胞膜定向，并终止于骨表面的短突起。这些细丝也与HMM反应。细丝相对于骨细胞的生理可能的意义进行了讨论。
A variety of intracellular filaments (50-70 A in diameter) found in bone cells was shown to bind specifically to HMM. Because of this property, these filaments are probably biochemically similar to muscle actin. in osteoblasts and osteocytes, these reactive filaments were oriented in bundles parallel to the plasma membrane and filling the cell processes. In the osteoclast the filaments along the cell membrane were not so highly organized. In the clear zone, the quiescent part of the cell adjacent to the motile ruffled border, organized filament bundles were oriented perpendicular to the cell membrane and terminated in short processes at the bone surface. These filaments were also reactive with HMM. The possible significance of the filaments with respect to the physiology of bone cells is discussed.