Nuclear receptors in Bombyx mori: Insights into genomic structure and developmental expression
Nuclear receptors in Bombyx mori: Insights into genomic structure and developmental expression
复制标题
家蚕核受体：深入了解基因组结构和发育表达
DOI：
10.1016/j.ibmb.2008.09.013
复制
发表时间：
2008-12-01
影响因子：
3.8
通讯作者：
Xiang, Zhonghuai
中科院分区：
文献类型：
--
作者：
Cheng, Daojun;Xia, Qingyou;Xiang, Zhonghuai
核受体（NR）作为配体依赖性转录因子发挥作用，并参与不同动物的多种生物学过程。更新的家蚕全基因组序列的组装，使包括B在内的5种全变态昆虫的NR的系统分析成为可能。mori、黑腹果蝇、冈比亚按蚊、意大利蜜蜂和赤拟谷盗。因此，在B中识别出19个NR。在家蚕基因组中，18个NR与其他4种昆虫的同源性为1：11：1。有趣的是，B. mori为2.4，远高于其他4种昆虫;各NR的直向同源物LBD中内含子的基因组位置呈现出更大的多样性。这五种昆虫的所有NR的系统发育树与这些昆虫物种的经典系统发育一致或异常。所有NR在数量、基因组结构和进化上的特征揭示了它们在昆虫进化过程中的保守性和分化性。几个NR基因的表达模式表现出与D.可能与家蚕变态过程中的关键生物学过程有关。Bm β FTZ-F1的RNAi导致幼虫-蛹转变异常，进一步表明它在家蚕变态中也是至关重要的。总之，本研究为深入了解该基因的结构、进化、表达等方面提供了新的视角。和家蚕核受体的功能。(C)2008爱思唯尔有限公司保留所有权利。
Nuclear receptors (NRs) function as ligand-dependent transcription factors and are involved in diverse biological processes in different animals. The updated assembly of complete genome sequence of the Bombyx mori enabled a systematic analysis of the NRs in the five holometabolous insects including B. mori, Drosophila melanogaster, Anopheles gambiae, Apis mellifera, and Tribolium castaneum. As a result, nineteen NRs were identified in the B. mori genome, each of eighteen NRs has 1:11:1 ortholog in the other four insects. Interestingly, the average intron number of ligand-binding domain (LBD) of each NR gene in B. mori was 2.4, much higher than that in the other four insects; the genomic position of introns in LBDs of all orthologs for each NR presents more diversity. Phylogenetic trees of all NRs from the five insects were consistent or aberrant with classical phylogeny of these insect species. The characteristics in number, genomic structure and phylogeny of all NRs revealed their evolutionary conservation and divergence during insect evolution. The expression patterns of several NR genes displayed temporal specificity similar to that in D. melanogaster and may be associated with the key biological processes during silkworm metamorphosis. The RNAi of Bm beta FTZ-F1 resulted in abnormality in larva-pupa transition, further suggesting it is also crucial for silkworm metamorphosis. In conclusion, the present study provided new insights into the structure, evolution, expression. and functions of silkworm NRs. (C) 2008 Elsevier Ltd. All rights reserved.