The mouse RecA-like gene Dmc1 is required for homologous chromosome synapsis during meiosis
The mouse RecA-like gene Dmc1 is required for homologous chromosome synapsis during meiosis
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DOI：
10.1016/s1097-2765(00)80070-2
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发表时间：
1998-04-01
期刊：
影响因子：
16
通讯作者：
Morita, T
中科院分区：
文献类型：
--
作者：
Yoshida, K;Kondoh, G;Morita, T
小鼠Dmc1基因是大肠杆菌RecA同源基因，在减数分裂中特异性表达。当同源配对可能启动时，在瘦素-合子素精母细胞中检测到DMC1蛋白。在雄性小鼠中，靶向基因破坏显示生殖细胞减数分裂在早期合子期停止，随后是细胞凋亡。在缺乏Dmc1基因的雌性小鼠中，胚胎中正常的卵发生分化被流产，生殖细胞在成年卵巢中消失。对dmc1缺陷小鼠精细胞的减数分裂染色体分析显示，同源物之间没有正确配对，单价轴元件随机分布。然而，在极少数情况下，我们观察到非同源物之间的复杂配对。因此，小鼠Dmc1基因是减数分裂中染色体同源突触所必需的。
The mouse Dmc1 gene is an E. coli RecA homolog that is specifically expressed in meiosis. The DMC1 protein was detected in leptotene-to-zygotene spermatocytes, when homolog pairing likely initiates. Targeted gene disruption in the male mouse showed an arrest of meiosis of germ cells at the early zygotene stage, followed by apoptosis. In female mice lacking the Dmc1 gene, normal differentiation of oogenesis was aborted in embryos, and germ cells disappeared in the adult ovary. Meiotic chromosome analysis of Dmc1-deficient mouse spermatocytes revealed random spread of univalent axial elements without correct pairing between homologs. In rare cases, however, we observed complex pairing among nonhomologs. Thus, the mouse Dmc1 gene is required for homologous synapsis of chromosomes in meiosis.