Cdc42 is not essential for filopodium formation, directed migration, cell polarization, and mitosis in fibroblastoid cells
Cdc42 is not essential for filopodium formation, directed migration, cell polarization, and mitosis in fibroblastoid cells
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DOI：
10.1091/mbc.e05-01-0061
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发表时间：
2005-10-01
影响因子：
3.3
通讯作者：
Brakebusch, C
中科院分区：
文献类型：
--
作者：
Czuchra, A;Wu, XW;Brakebusch, C
Cdc42是一种小的GT3，参与细胞骨架和细胞极性的调节。为了测试Cdc42是否在丝状伪足的形成或定向细胞迁移中具有重要作用，我们通过条件性基因失活产生Cdc42缺陷的成纤维样细胞。我们在这里报告，Cdc42的损失并不影响丝状伪足或板状伪足的形成，并没有显着的影响定向迁移的速度，也没有对有丝分裂。Cdc42缺陷细胞显示出更细长的细胞形状，并减少了面积。此外，迁移过程中的方向性和重新定位的高尔基体迁移的方向减少。然而，显性负Cdc42在Cdc42空细胞的表达导致强烈减少定向迁移，严重降低单细胞方向性，并完全丧失高尔基体极化和朝向伤口的突起形成的方向性，以及膜起泡。因此，我们的数据表明，除了Cdc42额外的GTP酶的Rho家族，共享GEFs与Cdc42，参与建立和维持细胞极性在定向迁移。
Cdc42 is a small GTPase involved in the regulation of the cytoskeleton and cell polarity. To test whether Cdc42 has an essential role in the formation of filopodia or directed cell migration, we generated Cdc42-deficient fibroblastoid cells by conditional gene inactivation. We report here that loss of Cdc42 did not affect filopodium or lamellipodium formation and had no significant influence on the speed of directed migration nor on mitosis. Cdc42-deficient cells displayed a more elongated cell shape and had a reduced area. Furthermore, directionality during migration and reorientation of the Golgi apparatus into the direction of migration was decreased. However, expression of dominant negative Cdc42 in Cdc42-null cells resulted in strongly reduced directed migration, severely reduced single cell directionality, and complete loss of Golgi polarization and of directionality of protrusion formation toward the wound, as well as membrane blebbing. Thus, our data show that besides Cdc42 additional GTPases of the Rho-family, which share GEFs with Cdc42, are involved in the establishment and maintenance of cell polarity during directed migration.