Increased circulating endothelial microparticles in COPD patients: a potential biomarker for COPD exacerbation susceptibility
Increased circulating endothelial microparticles in COPD patients: a potential biomarker for COPD exacerbation susceptibility
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DOI：
10.1136/thoraxjnl-2011-201395
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发表时间：
2012-12-01
期刊：
影响因子：
10
通讯作者：
Kubo, Hiroshi
中科院分区：
文献类型：
--
作者：
Takahashi, Toru;Kobayashi, Seiichi;Kubo, Hiroshi
COPD急性加重对内皮细胞的影响尚未完全了解。循环内皮微粒（circulating endothelial microparticles，EMPs）是由活化或凋亡的内皮细胞在循环血液中脱落的膜囊泡，目的比较稳定期COPD患者、急性加重期COPD患者和急性加重期COPD患者的EMPs数量。EMP定义为通过FACS分析的CD 144 + MP（VE-钙粘蛋白EMP）、CD 31 +/CD 41-MP（PECAM EMP）、CD 146 MP（MCAM EMP）和CD 62 E + EMP（E-选择素EMP）。结果稳定期COPD患者血清中VE-钙粘蛋白、PECAM和E-选择素EMP含量均显著高于非COPD组，加重期COPD患者血清中VE-钙粘蛋白、PECAM和E-选择素EMP含量均显著高于稳定期COPD组。大多数这些增加的EMP是vWF阴性的，表明肺毛细血管起源。COPD频繁加重患者的基线E-选择素EMP水平显著高于无频繁加重的患者（p < 0.001）。在急性发作后28天，E-选择素EMP水平恢复到稳定型COPD患者中观察到的水平，而PECAM EMP水平仍然很高。MCAM EMP数没有升高，在稳定或加重COPD patient.Conclusions内皮损伤，主要是在肺毛细血管，发生在急性加重期，并继续临床症状消失后。较高的基线E-选择素EMP水平可能表明COPD患者易发生急性加重。
Rationale The influence of COPD exacerbation on the endothelium is not completely understood. Circulating endothelial microparticles (EMPs) are membrane vesicles in circulating blood that are shed by activated or apoptotic endothelial cells.Objective To compare EMP numbers in stable COPD patients with those during and after exacerbation.Methods We examined the EMP numbers in 80 stable COPD patients, 27 patients with exacerbated COPD, and 20 healthy non-COPD volunteers. EMPs were defined as CD144+ MPs (VE-cadherin EMPs), CD31+/CD41-MPs (PECAM EMPs), CD146 MPs (MCAM EMPs) and CD62E + EMPs (E-selectin EMPs) as analysed by FACS. Von Willebrand factor (vWF) expression was utilised to identify the origins of the EMPs.Results VE-cadherin, PECAM and E-selectin EMP numbers were significantly higher in the stable COPD patients than in the non-COPD volunteers, and they were significantly higher in the patients with exacerbated COPD than in the stable COPD patients. The majority of these increased EMPs were vWF-negative, indicating a pulmonary capillary origin. Baseline E-selectin EMP levels were significantly higher in COPD patients who experienced frequent exacerbations than in those who did not have frequent exacerbations (p < 0.001). Twenty-eight days after the onset of exacerbation, E-selectin EMP levels returned to those observed in stable COPD patients, whereas PECAM EMP levels remained high. MCAM EMP numbers were not elevated in stable or exacerbated-COPD patients.Conclusions Endothelial damage, mainly in pulmonary capillaries, occurs during exacerbation and continues even after clinical symptoms disappear. Higher baseline E-selectin EMP levels may indicate COPD patients who are susceptible to exacerbation.