DIRECT LIQUID-CHROMATOGRAPHIC SEPARATION OF ENANTIOMERS ON IMMOBILIZED PROTEIN STATIONARY PHASES .4. MOLECULAR INTERACTION FORCES AND RETENTION BEHAVIOR IN CHROMATOGRAPHY ON BOVINE SERUM-ALBUMIN AS A STATIONARY PHASE
DIRECT LIQUID-CHROMATOGRAPHIC SEPARATION OF ENANTIOMERS ON IMMOBILIZED PROTEIN STATIONARY PHASES .4. MOLECULAR INTERACTION FORCES AND RETENTION BEHAVIOR IN CHROMATOGRAPHY ON BOVINE SERUM-ALBUMIN AS A STATIONARY PHASE
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DOI：
10.1016/s0021-9673(00)96187-9
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发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
BOREN, H
中科院分区：
文献类型：
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作者：
ALLENMARK, S;BOMGREN, B;BOREN, H
使用固定牛血清白蛋白作为固定相和水性缓冲系统作为洗脱剂的色谱法已被证明是一种高度选择性的方法，能够分离结构非常密切相关的化合物。通过改变流动相的至少三个独立参数可以有效地调节保留，这可以用于分离因子的优化。特别是，手性固定相的对映选择性性质已被证明可用于将多种外消旋体分析拆分为对映体。从保留行为随取代基效应的变化以及流动相组成的变化，已经获得了关于调节底物-蛋白质平衡的分子相互作用力的一些迹象。
Chromatography with the use of immobilized bovine serum albumin as a stationary phase and aqueous buffer systems as eluents has proved to be a highly selective method, capable of separating structurally very closely related compounds. Retention can be effectively regulated by changes in at least three independent parameters of the mobile phase, which may be used for an optimization of separation factors. Particularly, the enantioselective properties of the chiral stationary phase have been demonstrated to be useful for the analytical resolution of a variety of racemates into enantiomers. From the variation of the retention behaviour with substituent effects, as well as the mobile phase composition, some indications regarding the molecular interaction forces regulating the substrate—protein equilibria have been obtained.