A chemiluminescent probe specific for singlet oxygen.
A chemiluminescent probe specific for singlet oxygen.
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一种针对单线态氧的化学发光探针。
DOI：
10.1016/0006-291x(84)90311-5
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发表时间：
1984
影响因子：
3.1
通讯作者：
Thompson,A
中科院分区：
文献类型：
--
作者：
Posner,GH;Lever,JR;Miura,K;Lisek,C;Seliger,HH;Thompson,A
我们合成了一种甲氧基乙烯芘（MVP），以模拟观察到的苯并[a]芘7,8-二氢二醇（苯并[a]芘的近似致癌代谢物）微粒体化学发光的机制。该MVP类似物已被发现是一种高效和特异性的化学发光探针，用于测量单线态氧和单线态氧当量的皮摩尔量，并且当与细胞色素P-450酶反应时产生显著的化学发光。
We have synthesized a methoxyvinylpyrene (MVP) in order to model the mechanism for the observed microsomal chemiluminescence of benzo[a]pyrene 7,8-dihydrodiol, the proximate carcinogenic metabolite of benzo[a]pyrene. This MVP analog has been found to be a highly efficient and specific chemiluminescent probe for picomole quantities of singlet oxygen and singlet oxygen equivalents, and it produces significant chemiluminescence when reacted with cytochrome P-450 enzymes.