Directed bioconversion of Kraft lignin to polyhydroxyalkanoate by Cupriavidus basilensis B-8 without any pretreatment
Directed bioconversion of Kraft lignin to polyhydroxyalkanoate by Cupriavidus basilensis B-8 without any pretreatment
复制标题
无需任何预处理，Cupriavidus basilensis B-8 将硫酸盐木质素定向生物转化为聚羟基脂肪酸酯
DOI：
10.1016/j.procbio.2016.10.004
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发表时间：
2017-01-01
影响因子：
4.4
通讯作者：
Yuan, Joshua
中科院分区：
文献类型：
--
作者：
Shi, Yan;Yan, Xu;Yuan, Joshua
这项工作为硫酸盐木质素(KL)的生物转化提供了一种新的基本策略。巴西铜绿假单胞菌B-8(这里是B-8之后)能够利用KL作为唯一碳源。培养7d后，木质素、总碳(TC)和色度的脱色率分别达到41.5%、37.7%和43.0%。同时，通过扫描电子显微镜(SEM)和凝胶渗透色谱(GPC)证实了木质素的解聚为小碎片。当菌体初始KL浓度为5g L-1时，菌体生物量达到735.6 mg/L，相应的聚羟基烷酸的体积产率为128 mg/L，通过补料分批发酵，聚羟基烷酸的产量显著提高，达到319.4 mg/L。GC-MS分析表明，聚羟基烷酸聚合物由三种基本单体组成：98.3mol%的(S)-3-羟基丁酸(S3HB)、1.3mo%的(R)-ID3-羟基丁酸(R3HB)和0.4mol%的3-羟基丁酸(3HB)。(C)爱思唯尔有限公司出版的2016年。
This work presents here a new fundamental strategy for bio-converting Kraft lignin (KL) into useful products. Cupriavidus basilensis B-8 (here after B-8) was able to use KL as the sole carbon source. Fully 41.5% of lignin, 37.7% of total carbon (TC) and 43.0% of color were removed after 7 days of incubation. At the same time, lignin was depolymerized into small fragments, which was confirmed by scanning electron microscopy (SEM) and gel permeation chromatography (GPC). Bacterial biomass accumulated to 735.6 mg/L at the initial KL concentration of 5 g L-1, and the corresponding volumetric productivity of polyhydroxyalkanoate (PHA) was 128 mg/L. PHA productivity was significantly improved through fed batch fermentation and reached to 319.4 mg/L. GC-MS analysis showed that PHA polymer was composed of three basic monomers: 98.3 mol% of (S)-3-hydroxy-butanoic acid (S3HB), 1.3 mol% of (R) - ID3-hydroxybutyric acid (R3HB) and 0.4 mol% of 3-hydroxy-butanoic acid (3HB). (C) 2016 Published by Elsevier Ltd.