RNase H1, the Gold Standard for R-Loop Detection.
RNase H1, the Gold Standard for R-Loop Detection.
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DOI：
10.1007/978-1-0716-2477-7_7
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发表时间：
2022-01-01
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影响因子：
--
通讯作者：
Crouch, Robert J
中科院分区：
文献类型：
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作者：
Cerritelli, Susana M;Sakhuja, Kiran;Crouch, Robert J
RNaseH1已经成为揭示R环的生理和病理作用的重要工具，R环是由与单链DNA相反的RNA-DNA杂化结构组成的三链结构。RNaseH1降解R环的RNA部分，使两条DNA链变成双链形式(DsDNA)。RNaseH1在不同系统中的过表达有助于解决R环的位置、丰度以及形成、稳定和降解的机制等问题。在本章中，我们回顾了使用RNaseH1作为工具来研究R环的多种功能及其在健康和疾病中的相关性的多项研究。
RNase H1 has become an essential tool to uncover the physiological and pathological roles of R-loops, three-stranded structures consisting of and RNA-DNA hybrid opposite to a single DNA strand (ssDNA). RNase H1 degrades the RNA portion of the R-loops returning the two DNA strands to double-stranded form (dsDNA). Overexpression of RNase H1 in different systems has helped to address the questions of where R-loops are located, their abundance, and mechanisms of formation, stability, and degradation. In this chapter we review multiple studies that used RNase H1 as an instrument to investigate R-loops multiple functions and their relevance in health and diseases.