Transport of activated fatty acids by the peroxisomal ATP-binding-cassette transporter Pxa2 in a semi-intact yeast cell system.
Transport of activated fatty acids by the peroxisomal ATP-binding-cassette transporter Pxa2 in a semi-intact yeast cell system.
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在半完整酵母细胞系统中，过氧化物酶体 ATP 结合盒转运蛋白 Pxa2 转运活化脂肪酸。
DOI：
10.1111/j.1432-1033.1997.00657.x
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
R. Wanders
中科院分区：
文献类型：
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作者：
Nicolette Verleur;E. Hettema;C. V. Roermund;H. Tabak;R. Wanders
在酿酒酵母中，脂肪酸β-氧化仅限于过氧化物酶体。以前的研究已经表明脂肪酸进入过氧化物酶体的两种可能途径。第一种途径涉及中链脂肪酸作为游离脂肪酸穿过过氧化物酶体膜的运输，然后通过酰基辅酶A合成酶Faa 2 p在过氧化物酶体内活化。第二条途径涉及长链脂肪酸的运输。长链脂肪酸通过涉及在过氧化物酶体外空间中活化的途径进入过氧化物酶体，然后通过过氧化物酶体膜运输。有人认为，这种运输是依赖于过氧化物酶体ATP结合盒转运蛋白Pxa 1 p和Pxa 2 p。在本文中，我们调查是否Pxa 2 p是直接负责运输的C18：1-CoA，长链酰基辅酶A酯。使用原生质体，其中质膜已被选择性地渗透毛地黄皂苷，我们表明，C18：1-CoA，但不是C8：0-CoA，进入过氧化物酶体通过Pxa 2 p，在ATP依赖的方式。所获得的结果可能有助于阐明人类疾病X-连锁肾上腺脑白质营养不良的主要缺陷。
In the yeast Saccharomyces cerevisiae, fatty acid beta-oxidation is restricted to peroxisomes. Previous studies have shown two possible routes by which fatty acids enter the peroxisome. The first route involves transport of medium-chain fatty acids across the peroxisomal membrane as free fatty acids, followed by activation within the peroxisome by Faa2p, an acyl-CoA synthetase. The second route involves transport of long-chain fatty acids. Long-chain fatty acids enter the peroxisome via a route that involves activation in the extraperoxisomal space, followed by transport across the peroxisomal membrane. It has been suggested that this transport is dependent upon the peroxisomal ATP-binding-cassette transporters Pxa1p and Pxa2p. In this paper we investigated whether Pxa2p is directly responsible for the transport of C18:1-CoA, a long-chain acyl-CoA ester. Using protoplasts in which the plasma membrane has been selectively permeabilised by digitonin, we show that C18:1-CoA, but not C8:0-CoA, enters the peroxisome via Pxa2p, in an ATP-dependent fashion. The results obtained may contribute to the elucidation of the primary defect in the human disease X-linked adrenoleukodystrophy.