Suppression of tumor growth by intra-muscular transfer of naked DNA encoding adrenomedullin antagonist
Suppression of tumor growth by intra-muscular transfer of naked DNA encoding adrenomedullin antagonist
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DOI：
10.1038/sj.cgt.7700979
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发表时间：
2007-01-01
影响因子：
6.4
通讯作者：
Kobayashi, M.
中科院分区：
文献类型：
--
作者：
Miseki, T.;Kawakami, H.;Kobayashi, M.
我们最近报道，肾上腺髓质素（AM）拮抗剂（AMA; AM（22-52））肽的肿瘤内注射显着减少胰腺癌细胞系在严重联合免疫缺陷（SCID）小鼠的体内生长。在本研究中，我们研究了肿瘤内和肌肉内转移编码AMA的裸DNA对癌细胞系体内生长的影响。我们证明，这些治疗诱导的胰腺癌细胞系和乳腺癌细胞系接种在SCID小鼠的消退。此外，治疗后，CD 31阳性细胞从肿瘤组织中完全消失，表明新血管形成被完全抑制。这些结果表明，肿瘤内或肌肉内转移编码AMA的裸DNA可能是治疗人类癌症的一种有前途的替代方式。
We have recently reported that the intra-tumoral injection of adrenomedullin ( AM) antagonist ( AMA; AM ( 22-52)) peptides significantly reduced the in vivo growth of a pancreatic cancer cell line in severely combined immunodeficient ( SCID) mice. In the present study, we examined the effects of intra-tumoral and intra-muscular transfers of naked DNA encoding AMA on the in vivo growth of cancer cell lines. We demonstrate that these treatments induce the regression of a pancreatic cancer cell line and a breast cancer cell line inoculated in SCID mice. Furthermore, CD31-positive cells disappear completely from tumor tissues, following treatment, indicating that neo-vascularization is entirely inhibited. These results suggest that the intra-tumoral or intra-muscular transfer of naked DNA encoding AMA might be a promising alternative modality for treating human cancers.