Induction of MafBx and Murf ubiquitin ligase mRNAs in rat skeletal muscle after LPS injection
Induction of MafBx and Murf ubiquitin ligase mRNAs in rat skeletal muscle after LPS injection
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DOI：
10.1016/s0014-5793(03)00505-2
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发表时间：
2003-06-05
期刊：
影响因子：
3.5
通讯作者：
Thissen, JPM
中科院分区：
文献类型：
--
作者：
Dehoux, MJM;van Beneden, RP;Thissen, JPM
MafBx 和 Murf 是两种新的诱导肌肉萎缩的大鼠 E3 泛素连接酶。我们的目标是研究脂多糖 (LPS) 注射（一种肌肉分解代谢模型）是否与 MafBx 和 Murf 表达增加相关。 LPS（750 杯/100 克体重）诱导 MafBx 和 Murf mRNA（12 小时后分别为 23 倍和 33 倍；P < 0.001）。还证明了肿瘤坏死因子-α mRNA 的瞬时诱导（21 倍；3 小时时 P < 0.001）和胰岛素样生长因子-I mRNA 的减少（50%；6 小时时 P < 0.001），这是泛素-蛋白酶体系统的两个潜在调节剂。总之，MafBx 和 Murf mRNA 在 LPS 的作用下上调，并且可能在观察到的肌肉蛋白水解中发挥作用。 (C) 2003 年由 Elsevier Science B.V. 代表欧洲生化学会联合会出版。
MafBx and Murf are two new rat E3 ubiquitin ligases induced in muscle atrophy. Our goal was to investigate whether lipopolysaccharide (LPS) injection, a model of muscle catabolism, is associated with increased expression of MafBx and Murf. LPS (750 mug/100 g body weight) induces MafBx and Murf mRNA (respectively, 23-fold and 33-fold after 12 h; P < 0.001). A transient induction of tumor necrosis factor-alpha mRNA (21-fold; P < 0.001 at 3 h) and a decrease of insulin like growth factor-I mRNA (50%; P < 0.001 at 6 h), two potential regulators of the ubiquitin-proteasome system were also demonstrated. In summary, MafBx and Murf mRNA are upregulated in response to LPS and might play a role in the muscle proteolysis observed. (C) 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.