Characterization of Oita virus 296/1972 of Rhabdoviridae isolated from a horseshoe bat bearing characteristics of both lyssavirus and vesiculovirus
Characterization of Oita virus 296/1972 of Rhabdoviridae isolated from a horseshoe bat bearing characteristics of both lyssavirus and vesiculovirus
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DOI：
10.1007/s00705-003-0271-x
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发表时间：
2004-06-01
影响因子：
2.7
通讯作者：
Kurata, T
中科院分区：
文献类型：
--
作者：
Iwasaki, T;Inoue, S;Kurata, T
大分病毒296/1972是1972年从野生马蹄蝠Rhinolophus cornutus (Temminck)的血液中分离出来的。我们研究了该病毒对小鼠的致病性，包括其组织学、免疫组织化学和超微结构特征以及核蛋白基因的完整序列。该病毒通过脑内途径引起小鼠致命性脑炎。小鼠的这种易感性一直持续到3周龄。使用脑内接种后存活的成年小鼠获得的恢复期血清进行的免疫组织化学分析显示，许多神经元的细胞质呈阳性，并且与正常和狂犬病病毒感染的小鼠脑组织对该抗血清没有交叉反应。超微结构分析揭示了神经元中存在许多子弹状且有包膜的病毒体。病毒颗粒的这些形态特征与弹状病毒科病毒的形态特征一致。从内质膜出芽表明该病毒与狂犬病毒有相似之处。对编码暂定核蛋白序列的 cDNA 进行的分子分析揭示了其与弹状病毒科病毒的同源性。该基因序列与从哺乳动物中分离的其他弹状病毒的基因序列的距离矩阵分析揭示了该病毒在感染哺乳动物的弹状病毒科系统发育树中的离散位置，我们将该病毒重新命名为大分弹状病毒。
Oita virus 296/1972 was isolated from the blood of a wild horseshoe bat, Rhinolophus cornutus (Temminck) in 1972. We investigated the pathogenicity of this virus in mice in relation to its histological, immunohistochemical and ultrastructural characteristics and the entire sequence of nucleoprotein gene. This virus caused lethal encephalitis in mice through intracerebral route. This susceptibility of mice was until 3 weeks of age. Immunohistochemical analysis using the convalescent sera obtained from survived adult mice after intracerebral inoculation revealed that many neurons were positive in the cytoplasm, besides no cross reactivity with normal and rabies virus-infected mouse brain tissues to this anti-sera. Ultrastructural analysis disclosed many bullet-shaped and enveloped virions in neurons. These morphological characteristics of the virions are consistent of that of viruses in the family Rhabdoviridae. Budding from endoplasmic membrane suggests that this virus has a similarity with lyssaviruses. Molecular analysis of cDNA coding a tentative nucleoprotein sequence revealed homology with those of viruses in the family Rhabdoviridae. Distance matrix analysis of this gene sequence with those of other rhabdoviruses isolated from mammals disclosed the discrete position of this virus in the phylogenic tree of rhabdoviridae infecting mammals and we renamed this virus as Oita rhabdovirus.