IMMUNOHISTOCHEMICAL LOCALIZATION OF CYTOCHROME-P450 3A IN HUMAN PULMONARY CARCINOMAS AND NORMAL BRONCHIAL TISSUE
IMMUNOHISTOCHEMICAL LOCALIZATION OF CYTOCHROME-P450 3A IN HUMAN PULMONARY CARCINOMAS AND NORMAL BRONCHIAL TISSUE
复制标题
DOI：
10.1007/bf01464472
复制
发表时间：
1995-01-01
影响因子：
2.3
通讯作者：
KROEMER, HK
中科院分区：
文献类型：
--
作者：
KIVISTO, KT;FRITZ, P;KROEMER, HK
细胞色素P450 （CYP）酶在肝脏和一些肝外组织中代谢药物和其他异种生物。我们研究了CYP3A在同一患者的原发性肺组织和正常肺组织中的表达和定位。本研究包括32例接受部分或全肺切除治疗原发性肺癌的患者。对CYP3A进行免疫组化染色，采用改良的ABC技术。32例原发性肺癌中有8例CYP3A表达。32例正常组织中12例血清粘液腺CYP3A阳性。支气管上皮CYP3A阳性11例。我们观察到CYP3A在肿瘤组织中的表达与浆液腺和支气管上皮的表达无相关性。我们得出结论，CYP3A存在于正常和癌肺组织中。然而，我们的研究结果表明，CYP3A在肺癌中没有共表达。
The cytochrome P450 (CYP) enzymes metabolize drugs and other xenobiotics in liver and also in some extrahepatic tissues. We have studied the expression and localization of CYP3A in primary lung rumours and normal lung tissue from the same patients. Thirty-two patients undergoing partial or total lung resection for therapy of primary pulmonary carcinoma were included in this study. Immunohistochemical staining for CYP3A was performed with a modification of the ABC technique. Eight of the 32 cases of primary pulmonary carcinoma showed expression of CYP3A. In 12 of the 32 cases of normal tissue, the seromucous glands were positive for CYP3A. The bronchial epithelium was positive for CYP3A in 11 cases. We observed no correlation between CYP3A expression in tumour tissue and that in seromucous glands or bronchial epithelium. We conclude that CYP3A is present in both normal and cancerous lung tissue. Our findings suggest, however, no co-expression of CYP3A in lung cancer.