Osteoclastogenic with age potential of bone marrow cells increases in elderly women with fracture
Osteoclastogenic with age potential of bone marrow cells increases in elderly women with fracture
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DOI：
10.1016/s0047-6374(02)00071-4
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发表时间：
2002-07-01
影响因子：
5.3
通讯作者：
Yamamoto, S
中科院分区：
文献类型：
--
作者：
Koshihara, Y;Suematsu, A;Yamamoto, S
老年女性骨量减少的最可靠解释是破骨细胞吸收相对增加导致破骨细胞吸收和成骨细胞形成不平衡。然而，目前尚不清楚破骨细胞骨吸收随年龄增加是由于破骨细胞形成增加还是破骨细胞骨吸收活性增加所致。在这项研究中，我们使用人类骨髓培养系统，试图阐明破骨细胞形成随年龄的增加。从患有骨折的女性老年患者股骨近端区域获得的富含单核细胞的骨髓部分在 1,25 二羟基维生素 D-3 存在下培养 14 天。将耐酒石酸酸性磷酸酶阳性多核细胞计数为破骨细胞。在我们的调查中，随着老年女性（64-96 岁）年龄的增长，骨髓培养中的人破骨细胞形成增加。破骨细胞的形成与骨髓培养中巨噬细胞集落刺激因子和前列腺素E的产生呈正相关。此外，破骨细胞的离体形成与腰椎（L2-L4）的骨矿物质密度呈负相关。上述结果表明，老年骨折女性的骨髓细胞破骨潜能随着年龄的增长而增加，提示老年女性骨量的减少可能是由于与衰老相关的破骨细胞数量的增加所致。 (C) 2002 Elsevier Science Ireland Ltd. 保留所有权利。
The most reliable explanation for decreasing bone mass in elderly women is an imbalance of osteoclastic resorption and osteoblastic formation resulting from a relative increase in osteoclastic resorption. However, it is not clear whether an increase in osteoclastic bone resorption with age is due to increased osteoclast formation or to osteoclastic bone resorption activity. In this study, using a human bone marrow culture system, we attempt to clarify the increase in osteoclast formation with age. The mononuclear cell-rich fraction from bone marrow, obtained from the proximal region of the femur from female elderly patients with fracture, were cultured for 14 days in the presence of 1,25 dihydroxyvitamin D-3. Tartrate-resistant acid phosphatase-positive multinucleated cells were counted as osteoclasts. In our investigation, human osteoclast formation in the bone marrow culture increased with age in elderly women (age 64-96 years). The osteoclast formation was positively correlated with macrophage-colony stimulation factor and prostaglandin E, production in bone marrow culture. Also, osteoclast formation ex vivo was negatively correlated with bone mineral density of the lumbar spine (L2-L4). The above results indicate that the osteoclastogenic potential of bone marrow cells increases with aging in elderly women with fracture, and suggest that a decrease in bone mass of elderly women may be due to an increase in osteoclast population associated with aging. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.