Contribution of plasma membrane lipid domains to red blood cell (re)shaping.
Contribution of plasma membrane lipid domains to red blood cell (re)shaping.
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DOI：
10.1038/s41598-017-04388-z
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发表时间：
2017-06-27
影响因子：
4.6
通讯作者：
Tyteca D
中科院分区：
文献类型：
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作者：
Leonard C;Conrard L;Guthmann M;Pollet H;Carquin M;Vermylen C;Gailly P;Van Der Smissen P;Mingeot-Leclercq MP;Tyteca D
尽管脂质结构域已在几种活细胞质膜中得到证实，但它们的作用仍不清楚。我们在这里研究了它们是否有助于功能相关的细胞（重新）塑造。为了解决这个问题，我们使用红细胞作为细胞模型，因为它们（i）表现出特定的双凹形状，允许血液循环中的可逆变形，而随着年龄的增长，这种变形会因膜囊泡而消失； (ii) 在其外质膜小叶上显示出两种类型的亚微米结构域，其中胆固醇和鞘磷脂的含量不同。我们在这里揭示了富含胆固醇和鞘磷脂的结构域与红细胞双凹膜的不同曲率区域的特定关联。红细胞变形后，富含胆固醇的区域聚集在高曲率区域。相比之下，在形状恢复过程中，富含鞘磷脂的结构域在钙流出时丰度增加。将红细胞储存在 4°C 下（模拟衰老）后，脂质域出现为特定的囊泡形成位点。总而言之，我们的数据表明脂质结构域可能有助于红细胞功能相关的（重新）塑造。
Although lipid domains have been evidenced in several living cell plasma membranes, their roles remain largely unclear. We here investigated whether they could contribute to function-associated cell (re)shaping. To address this question, we used erythrocytes as cellular model since they (i) exhibit a specific biconcave shape, allowing for reversible deformation in blood circulation, which is lost by membrane vesiculation upon aging; and (ii) display at their outer plasma membrane leaflet two types of submicrometric domains differently enriched in cholesterol and sphingomyelin. We here reveal the specific association of cholesterol- and sphingomyelin-enriched domains with distinct curvature areas of the erythrocyte biconcave membrane. Upon erythrocyte deformation, cholesterol-enriched domains gathered in high curvature areas. In contrast, sphingomyelin-enriched domains increased in abundance upon calcium efflux during shape restoration. Upon erythrocyte storage at 4 °C (to mimick aging), lipid domains appeared as specific vesiculation sites. Altogether, our data indicate that lipid domains could contribute to erythrocyte function-associated (re)shaping.