Picroside II Protects Rat Lung and A549 Cell Against LPS-Induced Inflammation by the NF-κB Pathway
Picroside II Protects Rat Lung and A549 Cell Against LPS-Induced Inflammation by the NF-κB Pathway
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Picroside II 通过 NF-kappa B 途径保护大鼠肺和 A549 细胞免受 LPS 诱导的炎症
DOI：
10.1007/s10753-017-0519-3
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发表时间：
2017-06-01
期刊：
影响因子：
5.1
通讯作者：
Feng, Haihua
中科院分区：
文献类型：
--
作者：
Shen, Bingyu;Zhao, Chenxu;Feng, Haihua
胡黄连苷II是中药胡黄连根部的主要活性成分，具有改善脑缺血、保护肝脏等保健作用。然而，其对急性肺损伤的影响尚不清楚。本研究的目的是评价胡黄连苷II对小鼠急性肺损伤和脂多糖（LPS）诱导的A549细胞炎症的影响。我们通过酶联免疫吸附试验（ELISA）和定量聚合酶链反应（qRT-PCR）评估了体内和体外肿瘤坏死因子-α（TNF-α）、白细胞介素-1（IL-1 β）和白细胞介素-6（IL-6）的水平。结果显示胡黄连苷II显著降低细胞和小鼠中TNF-α、IL-1 β和IL-6的浓度。Western blot和免疫荧光分析表明胡黄连苷II抑制了p65 NF-κ B信号通路的激活。在小鼠急性肺损伤模型中，胡黄连苷Ⅱ治疗后，肺组织病理改变减轻，肺湿/干重比值降低。总之，胡黄连苷II在细胞和动物中显示出有希望的抗炎作用。
Picroside II is the main active ingredient in the root department of Chinese medicine Picrorhiza scrophulariiflora which has been proved to have beneficial effects on health, such as ameliorating the cerebral ischemia and protecting the liver. However, its effects on acute lung injury remain unclear. The purpose of the study was to evaluate the effects of picroside II on acute lung injury in mice and the inflammation in A549 cells which are lipopolysaccharide (LPS) induced. We evaluated the levels of tumor necrosis factor-alpha (TNF-alpha), interleukin-1 (IL-1 beta), and interleukin-6 (IL-6) in vivo and vitro by enzyme-linked immunosorbent assay (ELISA) and quantitative polymerase chain reaction (qRT-PCR). Results showed picroside II significantly decreased the concentrations of TNF-alpha, IL-1 beta, and IL-6 in cells and mice. In addition, Western blot and immunofluorescence analysis indicated that picroside II suppressed the activation of p65 NF-kappa B signaling pathway compared with LPS stimulation. In the acute lung injury model of mice, after picroside II treatment, the pathologic changes of lung tissues had been alleviated and lung wet/dry weight ratio was decreased. In summary, picroside II showed the promising effects of anti-inflammation in cells and animals.