SERUM CONTAINS A PLATELET-DERIVED TRANSFORMING GROWTH-FACTOR
SERUM CONTAINS A PLATELET-DERIVED TRANSFORMING GROWTH-FACTOR
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DOI：
10.1073/pnas.79.17.5312
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发表时间：
1982-01-01
期刊：
影响因子：
--
通讯作者：
MOSES, HL
中科院分区：
文献类型：
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作者：
CHILDS, CB;PROPER, JA;MOSES, HL
高浓度胎牛血清诱导依赖于锚定的、未转化的小鼠[胚胎AKR-2B和大鼠NRK细胞]在软琼脂中形成克隆。胎牛血清中的集落刺激活性用45%饱和硫酸铵沉淀，并在分子筛层析中作为单一活性峰在10,000-15,000 mW范围内迁移。该菌落刺激活性对热和酸稳定，并被胰酶和二硫苏糖醇破坏，表明该活性是由于一种需要二硫键才具有生物活性的多肽。集落刺激活性与与表皮生长因子受体的结合没有竞争。等电聚焦显示等电点在4-5范围内的活性。血清中的集落刺激活性可能来源于血小板，因为贫血小板血浆和贫血小板血浆来源的血清活性很低，而牛和人血小板的酸/乙醇提取物具有很强的集落刺激活性。用Bio-Gel P-60对血小板提取物进行层析，发现AKR-2B的集落刺激活性峰值在12,000和20,000 mW范围内。血小板集落刺激活性的其他生物学和化学性质与血清活性相同。血清中存在一种血小板衍生生长因子(S)，其性质与转化生长因子相似。
High concentrations of fetal bovine serum induced colony formation in soft agar by anchorage-dependent, nontransformed mouse [embryonic AKR-2B and rat NRK cells. The colony-stimulating activity in fetal bovine serum was precipitated by 45% saturated ammonium sulfate and migrated in molecular sieve chromatography as a single peak of activity in the 10,000-15,000 MW range. The colony-stimulating activity was heat-and acid-stable and was destroyed by trypsin and dithiothreitol, indicating the activity was due to a polypeptide that required disulfide bonds for biological activity. No competition for binding to the epidermal growth factor receptor was associated with the colony-stimulating activity. Isoelectric focusing revealed activity in the pI [isoelectric point] 4-5 range. The colony-stimulating activity in serum appeared to be of platelet origin, because platelet-poor plasma and platelet-poor plasma-derived serum contained little activity, whereas acid/ethanol extracts of bovine and human platelets had potent colony-stimulating activity. Chromatography of platelet extracts on Bio-Gel P-60 revealed peaks of AKR-2B colony-stimulating activity in the 12,000 and 20,000 MW ranges. The other biological and chemical properties of the platelet colony-stimulating activity were the same as those for the serum activity. The presence in serum of a platelet-derived growth factor(s) with properties similar to those of the transforming growth factor is indicated.