Multifunctional Fluorescent Probe for Sequential Detections of Glutathione and Caspase-3 in Vitro and in Cells
Multifunctional Fluorescent Probe for Sequential Detections of Glutathione and Caspase-3 in Vitro and in Cells
复制标题
用于体外和细胞内谷胱甘肽和 Caspase-3 连续检测的多功能荧光探针
DOI：
10.1021/ac4014012
复制
发表时间：
2013-07-02
影响因子：
7.4
通讯作者：
Liang, Gaolin
中科院分区：
文献类型：
--
作者：
Huang, Rui;Wang, Xijun;Liang, Gaolin
在此，我们报道了一种新的“on - on”策略，该策略基于异硫氰酸荧光素纳米颗粒（FITC-NPs）的组装和拆解，用于用多功能荧光探针1连续检测谷胱甘肽（GSH）和半胱天冬酶-3 （Casp3）。理论研究揭示了潜在的机制，令人满意地解释了这种连续增强荧光的实验结果。利用该探针，我们还成功地成像了凋亡细胞中Casp3的活性。
Herein, we report a new "On-On" strategy based on the assembly and disassembly of fluorescein isothiocyanate nanoparticles (FITC-NPs) for sequential detections of glutathione (GSH) and caspase-3 (Casp3) with a multifunctional fluorescent probe 1. Theoretical investigations revealed the underlying mechanism that satisfactorily explained experimental results of such consecutive enhancements of fluorescence. Using this probe, we also successfully imaged the Casp3 activity in apoptotic cells.