Evans blue dye-enhanced capillary-resolution photoacoustic microscopy in vivo
Evans blue dye-enhanced capillary-resolution photoacoustic microscopy in vivo
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DOI：
10.1117/1.3251044
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发表时间：
2009-09-01
影响因子：
3.5
通讯作者：
Wang, Lihong V.
中科院分区：
文献类型：
--
作者：
Yao, Junjie;Maslov, Konstantin;Wang, Lihong V.
完整和连续的微血管网络成像对于广泛的生物医学应用至关重要。光声断层扫描可以提供高分辨率的微血管成像使用血红蛋白内的红细胞（红细胞）作为内源性造影剂。然而，间歇性红细胞在毛细血管内的流动导致不连续和片断的毛细血管图像。为了克服这个问题，我们使用埃文斯蓝（EB）染料作为体内光声成像的造影剂。EB具有很强的光吸收性，并通过与白蛋白的化学结合在血流中均匀分布。在EB的帮助下，完整和连续的微血管网络——尤其是毛细血管——被成像。EB离开血流的扩散动力学和EB-白蛋白复合物的清除动力学也进行了定量研究。(C) 2009光学仪器工程师学会。(DOI: 10.1117/1.3251044)
Complete and continuous imaging of microvascular networks is crucial for a wide variety of biomedical applications. Photoacoustic tomography can provide high resolution microvascular imaging using hemoglobin within red blood cells (RBCs) as an endogenic contrast agent. However, intermittent RBC flow in capillaries results in discontinuous and fragmentary capillary images. To overcome this problem, we use Evans blue (EB) dye as a contrast agent for in vivo photoacoustic imaging. EB has strong optical absorption and distributes uniformly in the blood stream by chemically binding to albumin. With the help of EB, complete and continuous microvascular networks-especially capillaries-are imaged. The diffusion dynamics of EB leaving the blood stream and the clearance dynamics of the EB-albumin complex are also quantitatively investigated. (C) 2009 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3251044]