An inexpensive meter to measure differences in electrical resistance in the rat vagina during the ovarian cycle
An inexpensive meter to measure differences in electrical resistance in the rat vagina during the ovarian cycle
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DOI：
10.1152/jappl.2001.91.2.667
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发表时间：
2001-08-01
影响因子：
3.3
通讯作者：
Peuler, JD
中科院分区：
文献类型：
--
作者：
Ramos, SD;Lee, JM;Peuler, JD
大鼠阴道壁的固有电阻在发情开始时显著升高，然后在卵巢周期的剩余时间内再次下降到低水平。因此，已经开发了专门的仪器来测量这种阻力(在几秒钟内)，只需将一个装有一对记录电极的小探头插入阴道(即MK-10A阻抗检查器和EC40发情周期监测器)。如本文所述，这两种仪器在监测个体周期方面比更费力的方法要方便得多，在这些方法中，阴道涂片检查角化(C)、有核(N)和白细胞(L)细胞的数量变化。然而，它们也很昂贵，而且它们的使用基本上没有在文献中被引用。因此，我们试图确定，专业电工常用的简单、廉价的电表(具有电阻测量能力)是否也能起到同样的作用。我们选择了一个标准的多功能电表(22-178型，RadioShack)，并用屏蔽可听设备(42-2387A型，RadioShack)的内部线路制成的导线连接到它上，其一个男性端子用作阴道探头。在阴道涂片显示细胞数量为C.N.L(发情早期典型)的大鼠中，电阻很高，为488+/-130k欧加(18只大鼠)。在阴道涂片显示所有其他可能的细胞分布的大鼠中，电阻(合并)要低得多(P<；0.05)，124+/-23千伏(32只大鼠)。因此，在评估雌性大鼠的发情状态时，容易获得、价格低廉的电子仪器可能是有用的，无论是为了提高繁殖效率和/或用于涉及此类信息所需的实验的其他目的。
The inherent electrical resistance of the rat vaginal wall rises markedly near the beginning of estrus and then falls again to low levels for the remainder of the ovarian cycle. Accordingly, special instruments have been developed to measure such resistances (within seconds) on simply inserting a small probe fitted with a pair of recording electrodes into the vagina (i.e., the MK-10A impedance checker and the EC40 estrus cycle monitor). As described herein, these two instruments are far more convenient for monitoring individual cycles than more laborious methods in which vaginal smears are inspected for changes in numbers of cornified (C), nucleated (N), and leukocytic (L) cells. However, they are also expensive and their use has essentially remained un-cited in the literature. Thus we sought to determine whether a simple, inexpensive electrical meter (with resistance-measuring capacity), as commonly used by professional electricians, would serve the same purpose. We chose a standard multifunctional meter (model 22-178, RadioShack) and attached leads to it fabricated from the internal wiring of a shielded audiocable (model 42-2387A, RadioShack), one male terminal of which was used as a vaginal probe. In rats from which vagina smears revealed cell numbers in the order of C. N. L (typical of early estrus) electrical resistances were high, 488 +/- 130 k Omega (18 rats). In rats from which vagina smears revealed all other possible cell distributions, electrical resistances (combined) were much lower (P < 0.05), 124 +/- 23 kV (32 rats). Thus readily accessible, inexpensive electrical meters may be useful in assessing the status of estrus in female rats, either to improve reproductive efficiencies and/or for other purposes involving experiments in which such information is desirable.